IgE antibody | huMaE11
A microtiter plate was coated overnight with Human Anti-Omaliizumab Antibody (clone AbD20669, HCA236) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of omalizumab (therapeutic reference product) or Human Anti-IgE Antibody, MCA6105 (omalizumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Omalizumab Antibody (clone AbD20669_hIgG1, HCA235P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
0.5 μg of Human Anti-IgE Antibody, MCA6105 (omalizumab biosimilar, research grade) and omalizumab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.
Size exclusion chromatography (SEC) of Human Anti-IgE Antibody, MCA6105 (omalizumab biosimilar, research grade) and omalizumab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl, 200 mM Arginine; flow rate: 200 μl/min; inj. volume [μl]: 3; temp.: 25 °C; detection: 280 nm; sample: mAb (1 mg/ml).
Human anti IgE (Omalizumab Biosimilar)
Anti-IgE Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug omalizumab (Xolair) for research use. It can be used in bioanalytical assays and for studying biological pathways affected by the drug.
- Product Type
- Monoclonal Antibody
- Clone
- huMaE11
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA6105 | E | 0.2 mg |
|
Human Anti-IgE Antibody, clone huMaE11 is a research grade biosimilar of the monoclonal antibody drug omalizumab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic antibody omalizumab.
Human Anti-IgE Antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-omalizumab antibodies, for example HCA235P and HCA236. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-omalizumab antibody in IgG1 format as reference standard, for example HCA235.
Omalizumab (Xolair) is a recombinant humanized IgG1 kappa monoclonal antibody used in the treatment of patients with moderate or severe asthma. Allergic asthma is mediated by immunoglobulin E (IgE) released by B cells in response to allergen. Circulating IgE binds to the high-affinity IgE Fc receptor (FcεRI) expressed on basophils and mast cells, triggering the release of histamine, leukotrienes and other mediators associated with the pathophysiology of asthma. Omalizumab is directed against the Fc region of human IgE. By binding to circulating IgE at the site of FcεRI binding, this therapeutic antibody prevents the interaction of IgE with its receptor thus limiting mediator release.
This product is NOT FOR THERAPEUTIC USE.
View a summary of supporting anti-omalizumab antibody products
Human Anti-IgE Antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-omalizumab antibodies, for example HCA235P and HCA236. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-omalizumab antibody in IgG1 format as reference standard, for example HCA235.
Omalizumab (Xolair) is a recombinant humanized IgG1 kappa monoclonal antibody used in the treatment of patients with moderate or severe asthma. Allergic asthma is mediated by immunoglobulin E (IgE) released by B cells in response to allergen. Circulating IgE binds to the high-affinity IgE Fc receptor (FcεRI) expressed on basophils and mast cells, triggering the release of histamine, leukotrienes and other mediators associated with the pathophysiology of asthma. Omalizumab is directed against the Fc region of human IgE. By binding to circulating IgE at the site of FcεRI binding, this therapeutic antibody prevents the interaction of IgE with its receptor thus limiting mediator release.
This product is NOT FOR THERAPEUTIC USE.
View a summary of supporting anti-omalizumab antibody products
Product Details
- Target Species
- Human
- Product Form
- Human IgG1 (kappa) antibody - liquid
- Preparation
- Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Proclin 300
- Immunogen
- Human IgE protein
- Purity
- >98% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Licensed Use
- For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
- Regulatory
- For research purposes only
Applications of IgE antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml |
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