IgD antibody | LE2

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Mouse anti Human IgD (Fc)

Product Type
Monoclonal Antibody
Clone
LE2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA644G
Datasheet
C
MSDS
0.2 mg loader

Mouse anti Human IgD (Fc) antibody, clone LE2 recognizes an epitope located in the Fc region the heavy chain of human immunoglobulin D (IgD). Human IgD existst in two forms, the major membrane bound form which is co-expressed with IgM on the surface of mature B-cells (Blattner et al. 1984) and a minor secreted form comprising about 0.25% total serum Ig (Rogentine et al. 1966). Membrane bound IgD bears predominantly the κ light chain whereas the secreted form has predominantly λ light chains (Preud'homme et al. 2000)

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified Human IgD (Fc).
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P01880 Related reagents
Entrez Gene
IGHD Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IgD antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/100 1/300
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Immunofluorescence

Product Specific References

References for IgD antibody

  1. Creasey, A.M. et al. (2003) Nonspecific immunoglobulin M binding and chondroitin sulfate A binding are linked phenotypes of Plasmodium falciparum isolates implicated in malaria during pregnancy.
    Infect Immun. 71 (8): 4767-71.
  2. Kolar, G.R. et al. (2007) A novel human B cell subpopulation representing the initial germinal center population to express AID.
    Blood. 109: 2545-52.
  3. Sun, Z. et al. (2005) Semi-extended solution structure of human myeloma immunoglobulin D determined by constrained X-ray scattering.
    J Mol Biol. 353: 155-73.
  4. Toly-Ndour, C. et al. (2011) Acute renal failure with lambda light chain-derived crystals in a patient with IgD myeloma.
    Nephrol Dial Transplant. 26: 3057-9.