Mouse anti Human IgD (Fc) antibody, clone LE2
recognizes an epitope located in the Fc region the heavy chain of human immunoglobulin D (IgD). Human IgD existst in two forms, the major membrane bound form which is co-expressed with IgM on the surface of mature B-cells (Blattner et al. 1984
) and a minor secreted form comprising about 0.25% total serum Ig (Rogentine et al. 1966
). Membrane bound IgD bears predominantly the κ light chain whereas the secreted form has predominantly λ light chains (Preud'homme et al. 2000
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Purified Human IgD (Fc).
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IgD antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Application Based External Images
Product Specific References
References for IgD antibody
Creasey, A.M. et al. (2003) Nonspecific immunoglobulin M binding and chondroitin sulfate A binding are linked phenotypes of Plasmodium falciparum isolates implicated in malaria during pregnancy.
Infect Immun. 71 (8): 4767-71.
Kolar, G.R. et al. (2007) A novel human B cell subpopulation representing the initial germinal center population to express AID.
Blood. 109: 2545-52.
Sun, Z. et al. (2005) Semi-extended solution structure of human myeloma immunoglobulin D determined by constrained X-ray scattering.
J Mol Biol. 353: 155-73.
Toly-Ndour, C. et al. (2011) Acute renal failure with lambda light chain-derived crystals in a patient with IgD myeloma.
Nephrol Dial Transplant. 26: 3057-9.
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