Mouse anti Human Interferon gamma antibody, clone D9D10 recognizes both natural and recombinant human interferon gamma, binding only to the dimeric (functional) form.
Mouse anti Human Interferon gamma antibody, clone D9D10 has been reported to neutralize the activity of natural and recombinant IFN gamma.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Natural human interferon-gamma.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3X63-Ag-8.653 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
protein import into nucleus, translocation
positive regulation of mesenchymal cell proliferation
negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
response to virus
interferon-gamma receptor binding
cell cycle arrest
regulation of insulin secretion
negative regulation of gene-specific transcription from RNA polymerase II promoter
positive regulation of interleukin-12 production
positive regulation of interleukin-23 production
positive regulation of peptidyl-serine phosphorylation of STAT protein
positive regulation of smooth muscle cell apoptosis
tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of nitric oxide biosynthetic process
positive regulation of osteoclast differentiation
negative regulation of smooth muscle cell proliferation
positive regulation of membrane protein ectodomain proteolysis
positive regulation of killing of cells of other organism
interferon-gamma-mediated signaling pathway
regulation of interferon-gamma-mediated signaling pathway
positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
positive regulation of fructose 1,6-bisphosphate metabolic process
positive regulation of vitamin D biosynthetic process
positive regulation of calcidiol 1-monooxygenase activity
negative regulation of metanephric nephron tubule epithelial cell differentiation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IFN Gamma antibody
|Flow Cytometry 1
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for IFN Gamma antibody
Andersson, U. et al. (1986) Phenotypic characterization of individual interferon-gamma-producing cells after OKT3 antibody activation.
Eur J Immunol. 16 (11): 1457-60.
Sandvig, S. et al. (1987) Gamma-interferon is produced by CD3+ and CD3- lymphocytes.
Immunol Rev. 97: 51-65.
Andersson U et al. (1988) Enumeration of IFN-gamma-producing cells by flow cytometry. Comparison with fluorescence microscopy.
J Immunol Methods. 112 (1): 139-42.
Horowitz, A. et al. (2010) NK cells as effectors of acquired immune responses: effector CD4+ T cell-dependent activation of NK cells following vaccination.
J Immunol. 185 (5): 2808-18.
Buggins, A.G. ety al. (2001) Microenvironment produced by acute myeloid leukemia cells prevents T cell activation and proliferation by inhibition of NF-kappaB, c-Myc, and pRb pathways.
J Immunol. 167: 6021-30.
Burton, O.T. et al. (2010) Roles for TGF-beta and programmed cell death 1 ligand 1 in regulatory T cell expansion and diabetes suppression by zymosan in nonobese diabetic mice.
J Immunol. 185: 2754-62.
Cox, K. et al. (2005) Plasmacytoid dendritic cells (PDC) are the major DC subset innately producing cytokines in human lymph nodes.
J Leukoc Biol. 78: 1142-52.
Newman, K.C. et al. (2006) Cross-talk with myeloid accessory cells regulates human natural killer cell interferon-gamma responses to malaria.
PLoS Pathog. 2: e118.
Horowitz, A. & Riley, E.M. (2013) Activation of human NK cells by Plasmodium-infected red blood cells.
Methods Mol Biol. 923: 447-64.