HtrA2 antibody | AB03/4E9
Mouse anti HTRA2
HtrA2 antibody recognizes the serine protease HtrA2-mitochondrial, also known as HtrA-like serine protease or Omi stress-regulated endoprotease.
HTRA2 Antibody with Control Lysate
HtrA2 antibody recognizes the serine protease HtrA2-mitochondrial, also known as HtrA-like serine protease or Omi stress-regulated endoprotease.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- AB03/4E9
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00701 | WB | 100 µl | ||||
| VMA00701KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human HtrA2 antibody recognizes the serine protease HtrA2-mitochondrial, also known as HtrA-like serine protease, Omi stress-regulated endoprotease, high temperature requirement protein A2 and serine protease 25.
Encoded by the HTRA2 gene, this serine protease has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of HTRA2 results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined (provided by RefSeq, Jul 2008).
Mouse anti Human HtrA2 antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the HTRA2 gene, this serine protease has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of HTRA2 results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined (provided by RefSeq, Jul 2008).
Mouse anti Human HtrA2 antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti HtrA2 detects a band of approximately 33 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 134-458 of human HtrA2
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- O43464 Related reagents
- Entrez Gene
- HTRA2 Related reagents
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0004252 serine-type endopeptidase activity
- GO:0006915 apoptosis
- GO:0005634 nucleus
- GO:0005758 mitochondrial intermembrane space
- GO:0005789 endoplasmic reticulum membrane
- GO:0006508 proteolysis
- GO:0006950 response to stress
- GO:0009898 internal side of plasma membrane
- GO:0031966 mitochondrial membrane
- GO:0035631 CD40 receptor complex
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of HtrA2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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