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HtrA2 antibody | AB03/4E9

Mouse anti HTRA2

Product Type
PrecisionAb Monoclonal
Clone
AB03/4E9
Isotype
IgG1
Format
Purified
Specificity
HtrA2

Product Code Applications Pack Size List Price Your Price Qty
VMA00701
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti Human HtrA2 antibody recognizes the serine protease HtrA2-mitochondrial, also known as HtrA-like serine protease, Omi stress-regulated endoprotease, high temperature requirement protein A2 and serine protease 25.

Encoded by the HTRA2 gene, this serine protease has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of HTRA2 results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined (provided by RefSeq, Jul 2008).

Mouse anti Human HtrA2 antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti HtrA2 detects a band of approximately 33 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 134-458 of human HtrA2
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
O43464
Entrez Gene
HTRA2
GO Terms
GO:0051082 unfolded protein binding
GO:0004252 serine-type endopeptidase activity
GO:0006915 apoptosis
GO:0005634 nucleus
GO:0005758 mitochondrial intermembrane space
GO:0005789 endoplasmic reticulum membrane
GO:0006508 proteolysis
GO:0006950 response to stress
GO:0009898 internal side of plasma membrane
GO:0031966 mitochondrial membrane
GO:0035631 CD40 receptor complex

VMA00701

170803

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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