HSPA2 antibody | AB02/2E2

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Mouse anti HSPA2

Product Type
PrecisionAb Monoclonal
Clone
AB02/2E2
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00848
Datasheet
WB
SDS
100 µl loader

Mouse anti HSPA2 antibody recognizes heat shock-related 70 kDa protein 2.

. HSPA2 is a molecular chapherone protein within the HSP70 family, and was originally identified as crucial for spermatogenesis. The protein is highly expressed in spermatocytes, and could be a useful selection marker for sperm with lowest levels of chromatin damage in assisted reproductive techniques for male factor infertility (Heidari et al. 2018). In selected populations of somatic cells, non-spermatogenic functions of HSPA2 appear to include cytoprotection, and protection of cells against thermal stress, although its expression is not induced by heat shock (Scieglinska and Krawczyk, 2015). A recent role for HSPA2 was also found in maintenance of skin homeostasis (Gogler-Pig?owska et al. 2018). Although there is a marked difference in expression pattern between some cancer types and corresponding noncancerous tissues, the role of HSPA2 in cancer is unclear (Scieglinska and Krawczyk, 2015).

Product Details

Target Species
Human
Western Blotting
Mouse anti HSPA2 antibody detects a band of approximately 71 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-279 of human HSPA2
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P54652
Entrez Gene
HSPA2
GO Terms
GO:0051082 unfolded protein binding
GO:0006986 response to unfolded protein
GO:0005524 ATP binding
GO:0007140 male meiosis
GO:0007286 spermatid development
GO:0009986 cell surface
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of HSPA2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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