HSP90B1 antibody

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Anti HSP90B1 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human HSP90B1 antibody (**VMA00655**) followed by detection with Goat anti Rat IgG:HRP (Mouse Adsorbed) (1/10,000, STAR72) and visualized on the ChemiDoc MP with 119 second exposure. Arrow points to HSP90B1 (molecular weight 100 kDa).

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Rat anti HSP90B1

Product Type: PrecisionAb Monoclonal
Isotype: IgG2a
Format: Purified
Specificity: HSP90B1

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VMA00655 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rat anti HSP90B1 antibody recognizes 94 kDa glucose-regulated protein, also known as GRP94, endoplasmin, stress-inducible tumor rejection antigen gp96 or heat shock protein 90 kDa beta member 1.

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15 (provided by RefSeq, Aug 2012).

Rat anti HSP90B1 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Rat anti HSP90B1 antibody detects a band of approximately 100 kDa in HEK293 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rat monoclonal antibody purified by affinity chromatograpy on Protein A from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Purified glucose regulated protein 94 (grp94) from chicken oviducts
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rat IgG:HRP (Mouse Adsorbed)	STAR72	C E P	0.5 mg	Log in	Get Quote
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Description	Goat anti Rat IgG:HRP (Mouse Adsorbed)

UniProt: P14625
Entrez Gene: HSP90B1
GO Terms: GO:0051082 unfolded protein binding; GO:0005509 calcium ion binding; GO:0001666 response to hypoxia; GO:0003723 RNA binding; GO:0006457 protein folding; GO:0005829 cytosol; GO:0051208 sequestering of calcium ion; GO:0005524 ATP binding; GO:0005788 endoplasmic reticulum lumen; View More GO Terms; GO:0005789 endoplasmic reticulum membrane; GO:0005792 microsome; GO:0006916 anti-apoptosis; GO:0015031 protein transport; GO:0019903 protein phosphatase binding; GO:0030433 ER-associated protein catabolic process; GO:0030496 midbody; GO:0031247 actin rod assembly; GO:0042470 melanosome; GO:0043666 regulation of phosphoprotein phosphatase activity; GO:0046790 virion binding; GO:0048471 perinuclear region of cytoplasm; GO:0050750 low-density lipoprotein particle receptor binding; GO:0071318 cellular response to ATP