HSP90B1 antibody

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Rat anti HSP90B1

HSP90B1 antibody recognizes 94 kDa glucose-regulated protein, also known as GRP94, endoplasmin, stress-inducible tumor rejection antigen gp96 or heat shock protein 90 kDa beta member 1.

Product Type
PrecisionAb™ Monoclonal
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00655 WB 100 µl
Rat anti HSP90B1 antibody recognizes 94 kDa glucose-regulated protein, also known as GRP94, endoplasmin, stress-inducible tumor rejection antigen gp96 or heat shock protein 90 kDa beta member 1.

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15 (provided by RefSeq, Aug 2012).

Rat anti HSP90B1 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Western Blotting
Rat anti HSP90B1 antibody detects a band of approximately 100 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Rat monoclonal antibody purified by affinity chromatograpy on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Purified glucose regulated protein 94 (grp94) from chicken oviducts
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Store undiluted at -20oC, avoiding repeated freeze thaw cycles
As supplied, 12 months from date of despatch

More Information

P14625 Related reagents
Entrez Gene
HSP90B1 Related reagents
GO Terms
GO:0051082 unfolded protein binding
GO:0005509 calcium ion binding
GO:0001666 response to hypoxia
GO:0003723 RNA binding
GO:0006457 protein folding
GO:0005829 cytosol
GO:0051208 sequestering of calcium ion
GO:0005524 ATP binding
GO:0005788 endoplasmic reticulum lumen
GO:0005789 endoplasmic reticulum membrane
GO:0005792 microsome
GO:0006916 anti-apoptosis
GO:0015031 protein transport
GO:0019903 protein phosphatase binding
GO:0030433 ER-associated protein catabolic process
GO:0030496 midbody
GO:0031247 actin rod assembly
GO:0042470 melanosome
GO:0043666 regulation of phosphoprotein phosphatase activity
GO:0046790 virion binding
GO:0048471 perinuclear region of cytoplasm
GO:0050750 low-density lipoprotein particle receptor binding
GO:0071318 cellular response to ATP
PrecisionAb™ is a trademark of Bio-Rad Laboratories
For research purposes only

Applications of HSP90B1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
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