Hsc70 antibody

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Human anti Hsc70

Hsc70 antibody recognizes heat shock protein family A (Hsp70) member 8 also known as LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70 or heat shock cognate protein 54.

Hsc70 Antibody with Control Lysate

Hsc70 antibody recognizes heat shock protein family A (Hsp70) member 8 also known as LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70 or heat shock cognate protein 54.

Product Type
PrecisionAb™ Monoclonal
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00600 WB 100 µl
VMA00600KT WB 2 Westerns
With Lysate 400µg
Human anti Human Hsc70 antibody recognizes the heat shock protein family A (Hsp70) member 8 also known as LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70 or heat shock cognate protein 54.

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene (provided by RefSeq, Aug 2011).

Human anti Human Hsc70 antibody detects a band of 73 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Human monoclonal antibody purified by metal chelate affinity chromatography from E. coli extract
Preparation
20μl Human monoclonal antibody purified by metal chelate affinity chromatography from E. coli extract
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human Hsc70 protein (aa 1-646)
Fusion Partners
XYZ

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P11142 Related reagents
Entrez Gene
HSPA8 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0030529 ribonucleoprotein complex
GO:0006457 protein folding
GO:0042623 ATPase activity, coupled
GO:0006986 response to unfolded protein
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006892 post-Golgi vesicle-mediated transport
GO:0007269 neurotransmitter secretion
GO:0009986 cell surface
GO:0016044 cellular membrane organization
GO:0016071 mRNA metabolic process
GO:0042470 melanosome
GO:0044419 interspecies interaction between organisms
GO:0061202 clathrin sculpted gamma-aminobutyric acid transport vesicle membrane
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Hsc70 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Human anti Hsc70 antibody detects a band of approximately 73 kDa in K562 cell lysates
Western Blotting
Human anti Hsc70 antibody detects a band of approximately 73 kDa in K562 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB