Hsc70 antibody

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Anti Hsc70 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Hsc70 antibody (**VMA00600**) followed by detection with Goat anti Human IgG F(ab')2:HRP (1/2,500, STAR126) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to Hsc70 (molecular weight 73 kDa).

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Human anti Hsc70

Product Type: PrecisionAb Monoclonal
Isotype: HuCAL Fab bivalent
Format: Purified
Specificity: Hsc70

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00600 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Human anti Human Hsc70 antibody recognizes the heat shock protein family A (Hsp70) member 8 also known as LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70 or heat shock cognate protein 54.

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene (provided by RefSeq, Aug 2011).

Human anti Human Hsc70 antibody detects a band of 73 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Human anti Hsc70 antibody detects a band of approximately 73 kDa in K562 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Human monoclonal antibody purified by metal chelate affinity chromatography from E. coli extract.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli-derived recombinant human Hsc70 protein (aa 1-646)
Fusion Partners: XYZ
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Human IgG F(ab')2:HRP	STAR126P	E WB	0.5 ml	Log in	Get Quote
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Description	Goat anti Human IgG F(ab')2:HRP

UniProt: P11142
Entrez Gene: HSPA8
GO Terms: GO:0005886 plasma membrane; GO:0005515 protein binding; GO:0030529 ribonucleoprotein complex; GO:0006457 protein folding; GO:0042623 ATPase activity, coupled; GO:0006986 response to unfolded protein; GO:0005829 cytosol; GO:0005524 ATP binding; GO:0006892 post-Golgi vesicle-mediated transport; View More GO Terms; GO:0007269 neurotransmitter secretion; GO:0009986 cell surface; GO:0016044 cellular membrane organization; GO:0016071 mRNA metabolic process; GO:0042470 melanosome; GO:0044419 interspecies interaction between organisms; GO:0061202 clathrin sculpted gamma-aminobutyric acid transport vesicle membrane