HPRT1 antibody

100% Secure

Mouse anti HPRT1

Mouse anti-human HPRT1 antibody recognizes hypoxanthine-guanine phosphoribosyltransferase, also known as HGPRTase. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Product Code Applications Pack Size List Price Quantity
100 µl loader

Mouse anti Human HPRT1 antibody recognizes HPRT1 also known as hypoxanthine-guanine phosphoribosyltransferase or HGPRTase.

The protein encoded by HPRT1 gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in HPRT1 result in Lesch-Nyhan syndrome (LNS) or gout (provided by RefSeq, Jun 2009).

Mouse anti Human HPRT1 antibody detects a band of 24 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Western Blotting
Anti HPRT1 detects a band of approximately 24 kDa in HeLa cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Purified recombinant fragment of human HPRT1 (aa1-218) expressed in E. Coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
As supplied, 12 months from date of despatch.

More Information

P00492 Related reagents
Entrez Gene
HPRT1 Related reagents
GO Terms
GO:0000287 magnesium ion binding
GO:0001975 response to amphetamine
GO:0004422 hypoxanthine phosphoribosyltransferase activity
GO:0005829 cytosol
GO:0006166 purine ribonucleoside salvage
GO:0006168 adenine salvage
GO:0006178 guanine salvage
GO:0007625 grooming behavior
GO:0019835 cytolysis
GO:0021756 striatum development
GO:0021895 cerebral cortex neuron differentiation
GO:0021954 central nervous system neuron development
GO:0032263 GMP salvage
GO:0032264 IMP salvage
GO:0042803 protein homodimerization activity
GO:0045964 positive regulation of dopamine metabolic process
GO:0046100 hypoxanthine metabolic process
GO:0046651 lymphocyte proliferation
GO:0048813 dendrite morphogenesis
GO:0051289 protein homotetramerization
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
For research purposes only

Applications of HPRT1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml
Op-in to our ecommunications and set your preferences to start receiving resources, protocols, product updates, special offers, and more.

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information

Online Payment Methods:

  1. Invoice / Purchase Order
  2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
credit cards accepted