hnRNP U antibody | AB01/4D10

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Mouse anti hnRNP U

Product Type
PrecisionAb Monoclonal
Clone
AB01/4D10
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00683
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human hnRNP U antibody recognizes the heterogeneous nuclear ribonucleoprotein U, also known as p120 nuclear protein, scaffold attachment factor A and SAFA.

The heterogeneous nuclear ribonucleoproteins (hnRNPs) are a ubiquitously-expressed family of RNA-binding proteins that contribute to alternative splicing, mRNA stabilization, and regulation of transcription and translation. hnRNP U is the largest member of the hnRNP family, and is predominantly found in the nucleus where it binds to pre-mRNA and single-stranded DNA and is involved with transcription and alternative splicing (Geuens et al. 2016). hnRNP U is required for cell viability, with a hypomorphic mutation causing early embryonic lethality in mice (Roshon and Ruley 2005). Epigenetic inactivation of one of the two female X chromosomes, equalizing gene expression with male mammals, also depends on hnRNP U activity (Hasegawa et al. 2010).

Product Details

Target Species
Human
Western Blotting
Mouse anti hnRNP U detects a band of approximately 123 kDa in HeLa cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 371-825 of human hnRNP U
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q00839
Entrez Gene
HNRNPU
GO Terms
GO:0005515 protein binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0001906 cell killing
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0030530 heterogeneous nuclear ribonucleoprotein complex
GO:0009986 cell surface
GO:0070934 CRD-mediated mRNA stabilization
GO:0070937 CRD-mediated mRNA stability complex
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of hnRNP U antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml