hnRNP U antibody | AB01/4D10
Mouse anti Human hnRNP U antibody recognizes the heterogeneous nuclear ribonucleoprotein U, also known as p120 nuclear protein, scaffold attachment factor A and SAFA.
The heterogeneous nuclear ribonucleoproteins (hnRNPs) are a ubiquitously-expressed family of RNA-binding proteins that contribute to alternative splicing, mRNA stabilization, and regulation of transcription and translation. hnRNP U is the largest member of the hnRNP family, and is predominantly found in the nucleus where it binds to pre-mRNA and single-stranded DNA and is involved with transcription and alternative splicing (Geuens et al. 2016). hnRNP U is required for cell viability, with a hypomorphic mutation causing early embryonic lethality in mice (Roshon and Ruley 2005). Epigenetic inactivation of one of the two female X chromosomes, equalizing gene expression with male mammals, also depends on hnRNP U activity (Hasegawa et al. 2010).
The heterogeneous nuclear ribonucleoproteins (hnRNPs) are a ubiquitously-expressed family of RNA-binding proteins that contribute to alternative splicing, mRNA stabilization, and regulation of transcription and translation. hnRNP U is the largest member of the hnRNP family, and is predominantly found in the nucleus where it binds to pre-mRNA and single-stranded DNA and is involved with transcription and alternative splicing (Geuens et al. 2016). hnRNP U is required for cell viability, with a hypomorphic mutation causing early embryonic lethality in mice (Roshon and Ruley 2005). Epigenetic inactivation of one of the two female X chromosomes, equalizing gene expression with male mammals, also depends on hnRNP U activity (Hasegawa et al. 2010).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti hnRNP U detects a band of approximately 123 kDa in HeLa cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 371-825 of human hnRNP U
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q00839
- Entrez Gene
- HNRNPU
- GO Terms
- GO:0005515 protein binding
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0001906 cell killing
- GO:0003677 DNA binding
- GO:0003723 RNA binding
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0005681 spliceosomal complex
- GO:0030530 heterogeneous nuclear ribonucleoprotein complex
- GO:0009986 cell surface
- GO:0070934 CRD-mediated mRNA stabilization
- GO:0070937 CRD-mediated mRNA stability complex
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of hnRNP U antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | 1/2000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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