hnRNP L antibody | OTI9G7

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Mouse anti hnRNP L

Product Type
PrecisionAb Monoclonal
Clone
OTI9G7
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00261
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human hnRNP L antibody recognizes the heterogeneous nuclear ribonucleoprotein L (hnRNP L).

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. This protein is present in the nucleoplasm as part of the hnRNP complex but hnRNP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since hnRNP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for HNRNPL (provided by RefSeq, Jul 2008).

Mouse anti Human hnRNP L antibody detects a band of 64 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti hnRNP L detects a band of approximately 64 kDa in K562 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 270-502 of human hnRNP L (NP_001524) produced in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P14866 Related reagents
Entrez Gene
HNRNPL Related reagents
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003723 RNA binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0030530 heterogeneous nuclear ribonucleoprotein complex
GO:0010843 promoter binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of hnRNP L antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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