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hnRNP A antibody

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Anti hnRNP A Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1
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Western blot analysis of whole cell lysates probed with hnRNP A antibody followed by detection with Goat anti Human IgG F(ab')2:HRP (1/2,500, STAR126) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to hnRNP A (molecular weight 34 kDa).

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Human anti hnRNP A

Product Type
PrecisionAb Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified
Specificity
hnRNP A

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Human anti Human hnRNP A antibody recognizes heterogeneous nuclear ribonucleoprotein A1, also known as putative heterogeneous nuclear ribonucleoprotein A1-like 3, helix-destabilizing protein, heterogeneous nuclear ribonucleoprotein A1B protein, heterogeneous nuclear ribonucleoprotein B2 protein, heterogeneous nuclear ribonucleoprotein core protein A1, hnRNP A1-like 3, hnRNP core protein A1, hnRNP core protein A1-like 3, nuclear ribonucleoprotein particle A1 protein, single-strand DNA-binding protein UP1, single-strand RNA-binding protein.

HNRNPA1 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by HNRNPA1 has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for HNRNPA1 but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites (provided by RefSeq, Jul 2008).

Human anti Human hnRNP A antibody detects a band of 34 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Human anti hnRNP A antibody detects a band of approximately 34 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Human monoclonal antibody purified by metal chelate affinity chromatography from E. coli extract.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human hnRNP A
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader
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Description Goat anti Human IgG F(ab')2:HRP

UniProt
P09651
Entrez Gene
HNRNPA1
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0003697 single-stranded DNA binding
GO:0003727 single-stranded RNA binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0005730 nucleolus
GO:0006405 RNA export from nucleus
GO:0030530 heterogeneous nuclear ribonucleoprotein complex
GO:0044419 interspecies interaction between organisms
GO:0051028 mRNA transport
GO:0051170 nuclear import
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