HNF4A antibody | OTI2H2

100% Secure


Mouse anti HNF4A

Mouse anti human HNF-4-alpha antibody recognizes hepatocyte nuclear factor 4-alpha, also known as HNF4A, HNF4alpha10/11/12, TCF-14, transcription factor 14 or transcription factor HNF-4. This antibody has been extensively validated for western blotting using whole cell lysates.

HNF4A Antibody with Control Lysate

Mouse anti human HNF-4-alpha antibody recognizes hepatocyte nuclear factor 4-alpha, also known as HNF4A, HNF4alpha10/11/12, TCF-14, transcription factor 14 or transcription factor HNF-4. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI2H2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00379 WB 100 µl
VMA00379KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human HNF4A antibody recognizes hepatocyte nuclear factor 4-alpha, also known as TCF-14, nuclear receptor subfamily 2 group A member 1 and transcription factor 14.

Encoded by the HNF4A gene, hepatocyte nuclear factor 4-alpha is a nuclear transcription factor which binds DNA as a homodimer. Hepatocyte nuclear factor 4-alpha controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. HNF4A may play a role in development of the liver, kidney, and intestines. Mutations in HNF4A have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of HNF4A results in multiple transcript variants encoding several different isoforms (provided by RefSeq, Apr 2012).

Mouse anti Human HNF4A antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to aa 1-315 of human HNF4A (NP_849180) produced in E.coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P41235 Related reagents
Entrez Gene
HNF4A Related reagents
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of HNF4A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti HNF4A detects a band of approximately 52 kDa in HepG2 cell lysates
Western Blotting
Anti HNF4A detects a band of approximately 52 kDa in HepG2 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB