HMB45 antibody | HMB-45
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Mouse anti Human HMB45 antibody, clone HMB-45 recognizes the HMB-45 antigen, an intracytoplasmic antigen expressed by the majority of melanomas and with other tumors demonstrating melanocytic differentiation. tumors of lymphoid, epithelial, glial and mesenchymal origin are negative.
Normal adult melanocytes do not label with this antibody, although fetal and neonatal melanocytes stain variably.
Normal adult melanocytes do not label with this antibody, although fetal and neonatal melanocytes stain variably.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Extract of pigmented melanoma metastases from lymph nodes.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- P40967
- Entrez Gene
- PMEL
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0005789 endoplasmic reticulum membrane
- GO:0005794 Golgi apparatus
- GO:0032438 melanosome organization
- GO:0032585 multivesicular body membrane
- GO:0042438 melanin biosynthetic process
- GO:0042470 melanosome
- Regulatory
- For research purposes only
Applications of HMB45 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 1/40 | ||
| Immunohistology - Paraffin | 1/40 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Immunohistology
- This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Histology Positive Control Tissue
- Melanoma
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for HMB45 antibody
-
Fonsatti, E. et al. (2007) Functional up-regulation of human leukocyte antigen class I antigens expression by 5-aza-2'-deoxycytidine in cutaneous melanoma: immunotherapeutic implications.
Clin Cancer Res. 13: 3333-8. -
Clements, D. et Al. (2009) Analysis of the oestrogen response in an angiomyolipoma derived xenograft model.
Endocr Relat Cancer. 16: 59-72. -
Ginger, R.S. et al. (2008) SLC24A5 encodes a trans-Golgi network protein with potassium-dependent sodium-calcium exchange activity that regulates human epidermal melanogenesis.
J Biol Chem. 283: 5486-95.
Further Reading
-
Racchi, C.E. et al. (1996) HMB-45. A review.
Applied Immunohistochemistry. 4: 73-85.
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