HMB45 antibody | HMB-45

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Mouse anti Human HMB45

Product Type: Monoclonal Antibody
Clone: HMB-45
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2146	C P			1 ml

Mouse anti Human HMB45 antibody, clone HMB-45 recognizes the HMB-45 antigen, an intracytoplasmic antigen expressed by the majority of melanomas and with other tumors demonstrating melanocytic differentiation. tumors of lymphoid, epithelial, glial and mesenchymal origin are negative.

Normal adult melanocytes do not label with this antibody, although fetal and neonatal melanocytes stain variably.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.1% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Immunogen: Extract of pigmented melanoma metastases from lymph nodes.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt: P40967 Related reagents
Entrez Gene: PMEL Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0005515 protein binding; GO:0016021 integral to membrane; GO:0005576 extracellular region; GO:0005789 endoplasmic reticulum membrane; GO:0005794 Golgi apparatus; GO:0032438 melanosome organization; GO:0032585 multivesicular body membrane; GO:0042438 melanin biosynthetic process; GO:0042470 melanosome
Regulatory: For research purposes only

Applications of HMB45 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen			1/40
Immunohistology - Paraffin			1/40

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology: This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue: Melanoma

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

Immunofluorescence

Product Specific References

References for HMB45 antibody

Racchi, C.E. et al. (1996) HMB-45. A review.
Applied Immunohistochemistry. 4: 73-85.
Clements, D. et Al. (2009) Analysis of the oestrogen response in an angiomyolipoma derived xenograft model.
Endocr Relat Cancer. 16: 59-72.
Ginger, R.S. et al. (2008) SLC24A5 encodes a trans-Golgi network protein with potassium-dependent sodium-calcium exchange activity that regulates human epidermal melanogenesis.
J Biol Chem. 283: 5486-95.
Fonsatti, E. et al. (2007) Functional up-regulation of human leukocyte antigen class I antigens expression by 5-aza-2'-deoxycytidine in cutaneous melanoma: immunotherapeutic implications.
Clin Cancer Res. 13: 3333-8.

Request a different product with the HMB45 specificity