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Hltf antibody | AB03/1C7

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Anti Hltf Antibody, clone AB03/1C7 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Hltf Antibody, clone AB03/1C7 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti Hltf Antibody, clone AB03/1C7 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with HLTF antibody (1/1,000, VMA00883) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to HLTF (molecular weight 117 kDa)
Anti Hltf Antibody, clone AB03/1C7 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of HLTF from Jurkat whole cell lysate. The Input lane is 10% input; the HLTF-IP lane is the immunoprecipitation with the HLTF antibody (2.5 µg, VMA00883); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using HLTF antibody (1/1000, VMA00883) followed by detection with HRP conjugated Goat anti Mouse IgG (1/20,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to HLTF.

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WB IP

Mouse anti Hltf

Product Type
PrecisionAb Monoclonal
Clone
AB03/1C7
Isotype
IgG1
Format
Purified
Specificity
Hltf

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Product Code Applications Pack Size List Price Your Price Qty
VMA00883
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SDS Safety Datasheet SDS
IP WB 100 µl loader
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loader

Mouse anti HLTF antibody recognizes helicase-like transcription factor, also known as HIP116, RNF80, SMARCA3, SNF2L3 and ZBU1.

HLTF is a multi-domain, multi-functional enzyme, but its primary role appears to be genome stability. HLTF achieves repair of damaged DNA by associating with replication forks and binding to DNA through its substrate recognition domain (SRD). Accordingly, inactivation of HLTF results in phenotypes that suggest replication stress (Poole and Cortez 2017). HLTF appears to function as a tumor suppressor gene, with silencing of the gene encoding HLTF found in several cancer types. In cancer cell lines, sensitivity or resistance to lysosomal autophagy inhibitors appears to be genetically determined by HLTF expression. In addition to cancer, HLTF has been implicated in inflammatory bowel disease, and in the infection of cells by HIV (Elserafy et al. 2018).		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti HLTF antibody detects a band of approximately 117 kDa in Jurkat cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 435-1009 of human HLTF
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q14527
Entrez Gene
HLTF
GO Terms
GO:0003677 DNA binding
GO:0004386 helicase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006350 transcription
GO:0008270 zinc ion binding
GO:0016568 chromatin modification
GO:0016874 ligase activity

VMA00883

100004568

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