Hltf antibody | AB03/1C7

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Anti Hltf Antibody, clone AB03/1C7 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Hltf Antibody, clone AB03/1C7 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Hltf Antibody, clone AB03/1C7 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with HLTF antibody (1/1,000, **VMA00883**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to HLTF (molecular weight 117 kDa)

Anti Hltf Antibody, clone AB03/1C7 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of HLTF from Jurkat whole cell lysate. The Input lane is 10% input; the HLTF-IP lane is the immunoprecipitation with the HLTF antibody (2.5 µg, **VMA00883**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using HLTF antibody (1/1000, **VMA00883**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/20,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to HLTF.

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Mouse anti Hltf

Product Type: PrecisionAb Monoclonal
Clone: AB03/1C7
Isotype: IgG1
Format: Purified
Specificity: Hltf

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VMA00883 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti HLTF antibody recognizes helicase-like transcription factor, also known as HIP116, RNF80, SMARCA3, SNF2L3 and ZBU1.

HLTF is a multi-domain, multi-functional enzyme, but its primary role appears to be genome stability. HLTF achieves repair of damaged DNA by associating with replication forks and binding to DNA through its substrate recognition domain (SRD). Accordingly, inactivation of HLTF results in phenotypes that suggest replication stress (Poole and Cortez 2017). HLTF appears to function as a tumor suppressor gene, with silencing of the gene encoding HLTF found in several cancer types. In cancer cell lines, sensitivity or resistance to lysosomal autophagy inhibitors appears to be genetically determined by HLTF expression. In addition to cancer, HLTF has been implicated in inflammatory bowel disease, and in the infection of cells by HIV (Elserafy et al. 2018).

Target Species: Human
Western Blotting: Mouse anti HLTF antibody detects a band of approximately 117 kDa in Jurkat cell lysates
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 435-1009 of human HLTF
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q14527
Entrez Gene: HLTF
GO Terms: GO:0003677 DNA binding; GO:0004386 helicase activity; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0006350 transcription; GO:0008270 zinc ion binding; GO:0016568 chromatin modification; GO:0016874 ligase activity