Hltf antibody | AB03/1C7

Mouse anti Hltf

Product Type
PrecisionAb Monoclonal
Clone
AB03/1C7
Isotype
IgG1
Format
Purified
Specificity
Hltf

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VMA00883
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Mouse anti HLTF antibody recognizes helicase-like transcription factor, also known as HIP116, RNF80, SMARCA3, SNF2L3 and ZBU1.

HLTF is a multi-domain, multi-functional enzyme, but its primary role appears to be genome stability. HLTF achieves repair of damaged DNA by associating with replication forks and binding to DNA through its substrate recognition domain (SRD). Accordingly, inactivation of HLTF results in phenotypes that suggest replication stress (Poole and Cortez 2017). HLTF appears to function as a tumor suppressor gene, with silencing of the gene encoding HLTF found in several cancer types. In cancer cell lines, sensitivity or resistance to lysosomal autophagy inhibitors appears to be genetically determined by HLTF expression. In addition to cancer, HLTF has been implicated in inflammatory bowel disease, and in the infection of cells by HIV (Elserafy et al. 2018).
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti HLTF antibody detects a band of approximately 117 kDa in Jurkat cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 435-1009 of human HLTF
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q14527
Entrez Gene
HLTF
GO Terms
GO:0003677 DNA binding
GO:0004386 helicase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006350 transcription
GO:0008270 zinc ion binding
GO:0016568 chromatin modification
GO:0016874 ligase activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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