HLA DQ antibody | SPV-L3

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Mouse anti Human HLA DQ

Product Type
Monoclonal Antibody
Clone
SPV-L3
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA379G C F FN* IP 0.2 mg
Mouse anti human HLA DQ antibody, clone SPV-L3 reacts with a monomorphic determinant of human class II HLA DQ molecules and strongly blocks cytotoxicity.

Distribution studies suggest that it is directed against DQ rather than DR antigens. Mouse anti human HLA DQ antibody, clone SPV-L3 has been reported to inhibit the activity of cytotoxic T cell clones.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01909 Related reagents
P01906 Related reagents
Entrez Gene
HLA-DQA2 Related reagents
HLA-DQA1 Related reagents
GO Terms
GO:0002504 antigen processing and presentation of peptide or polysaccharide antigen via MHC class II
GO:0042613 MHC class II protein complex
GO:0032395 MHC class II receptor activity
GO:0005887 integral to plasma membrane
GO:0005765 lysosomal membrane
GO:0005789 endoplasmic reticulum membrane
GO:0005794 Golgi apparatus
GO:0006955 immune response
GO:0010008 endosome membrane
GO:0031295 T cell costimulation
GO:0050852 T cell receptor signaling pathway
GO:0060333 interferon-gamma-mediated signaling pathway
Regulatory
For research purposes only

Applications of HLA DQ antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Functional Assays 1
Immunohistology - Frozen
Immunoprecipitation
  1. 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control MCA929 100 Tests F

Application Based External Images

Flow Cytometry

Functional Assays

Product Specific References

References for HLA DQ antibody

  1. Spits, H. et al. (1984) HLA-DC antigens can serve as recognition elements for human cytotoxic T lymphocytes.
    Eur J Immunol. 14 (4): 299-304.
  2. Bontrop, R. et al. (1986) Polymorphisms within the HLA-DR4 haplotypes. Various DQ subtypes detected with monoclonal antibodies
    Tissue Antigens 27: 22-31.
  3. Yang, P. et al. (2002) Immune cells in the porcine retina: distribution, characterization and morphological features.
    Invest Ophthalmol Vis Sci. 43 (5): 1488-92.
  4. Hake, S.B. et al. (2000) CIITA leucine-rich repeats control nuclear localization, in vivo recruitment to the major histocompatibility complex (MHC) class II enhanceosome, and MHC class II gene transactivation.
    Mol Cell Biol. 20: 7716-25.
  5. Long, H.M. et al. (2005) CD4+ T-cell responses to Epstein-Barr virus (EBV) latent-cycle antigens and the recognition of EBV-transformed lymphoblastoid cell lines.
    J Virol. 79: 4896-907.
  6. Peretti, M. et al. (2001) Expression of the three human major histocompatibility complex class II isotypes exhibits a differential dependence on the transcription factor RFXAP.
    Mol Cell Biol. 21: 5699-709.
  7. Krawczyk, M. et al. (2005) New functions of the major histocompatibility complex class II-specific transcription factor RFXANK revealed by a high-resolution mutagenesis study.
    Mol Cell Biol. 25: 8607-18.
  8. Henderson, K.N. et al. (2007) A structural and immunological basis for the role of human leukocyte antigen DQ8 in celiac disease.
    Immunity. 27: 23-34.
  9. Long, H.M. et al. (2011) Cytotoxic CD4+ T Cell Responses to EBV Contrast with CD8 Responses in Breadth of Lytic Cycle Antigen Choice and in Lytic Cycle Recognition.
    J Immunol. 187: 92-101.
  10. Tagawa, T. et al. (2016) Epstein-Barr viral miRNAs inhibit antiviral CD4+ T cell responses targeting IL-12 and peptide processing.
    J Exp Med. Sep 12. pii: jem.20160248. [Epub ahead of print]
  11. Mahon, N.G. et al. (2002) Immunohistologic evidence of myocardial disease in apparently healthy relatives of patients with dilated cardiomyopathy.
    J Am Coll Cardiol. 39 (3): 455-62.
  12. Gudgeon, N.H. et al. (2005) Regression of Epstein-Barr virus-induced B-cell transformation in vitro involves virus-specific CD8+ T cells as the principal effectors and a novel CD4+ T-cell reactivity.
    J Virol. 79 (9): 5477-88.
  13. Böckle BC et al. (2008) DC-sign+ CD163+ macrophages expressing hyaluronan receptor LYVE-1 are located within chorion villi of the placenta.
    Placenta. 29 (2): 187-92.
  14. Onion, D. et al. (2007) The CD4+ T-cell response to adenovirus is focused against conserved residues within the hexon protein.
    J Gen Virol. 88 (Pt 9): 2417-25.
  15. Chentoufi, A.A. et al. (2008) Asymptomatic human CD4+ cytotoxic T-cell epitopes identified from herpes simplex virus glycoprotein B.
    J Virol. 82 (23): 11792-802.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.