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HLA ABC antibody | W6/32

Mouse anti Human HLA ABC:Pacific Blue®

Product Type
Monoclonal Antibody
Clone
W6/32
Isotype
IgG2a
Specificity
HLA ABC

Product Code Applications Pack Size List Price Your Price Qty
MCA81PB
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
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Mouse anti Human HLA ABC antibody, clone W6/32 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.

Mouse anti Human HLA ABC antibody, clone W6/32 is routinely tested in flow cytometry on human peripheral blood lymphocytes.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Macaque
Bovine
Cynomolgus monkey
Baboon
Rhesus Monkey
Chimpanzee
Gorilla
Shrew
Goat
Dog
Guinea Pig
Rabbit
Mouse
Chicken
Amphibia
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Pacific Blue - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Purified human tonsil lymphocyte membranes.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Pacific Blue® 410 455
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:Pacific Blue® MCA929PB F 100 Tests/1ml loader
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List Price Your Price
loader
Description Mouse IgG2a Negative Control:Pacific Blue®

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

Source Reference

  1. Barnstable, C.J. et al. (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
    Cell. 14 (1): 9-20.

References for HLA ABC antibody

  1. Brodsky, F.M. & Parham, P. (1982) Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies.
    Immunogenetics. 15 (2): 151-66.
  2. Neefjes, J.J. et al. (1986) A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes.
    Immunogenetics. 23 (5): 341-7.
  3. Stern, P.L. et al. (1987) Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast.
    J Immunol. 138 (4): 1088-91.
  4. Jacobsen, C.N. et al. (1993) Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species.
    Vet Immunol Immunopathol. 39 (4): 461-6.
  5. Verbeek, M.M. et al. (1995) T lymphocyte adhesion to human brain pericytes is mediated via very late antigen-4/vascular cell adhesion molecule-1 interactions.
    J Immunol. 154 (11): 5876-84.
  6. Raftery, M.J. et al. (2002) Hantavirus infection of dendritic cells.
    J Virol. 76: 10724-33.
  7. Dressel, R. et al. (2003) Differential effect of acute and permanent heat shock protein 70 overexpression in tumor cells on lysability by cytotoxic T lymphocytes.
    Cancer Res. 63 (23): 8212-20.
  8. Ishitani, A. et al. (2003) Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition.
    J Immunol. 171 (3): 1376-84.
  9. View The Latest Product References
  10. Giuliani, F. et al. (2003) Vulnerability of human neurons to T cell-mediated cytotoxicity.
    J Immunol. 171: 368-79.
  11. Smith, A.C. et al. (2005) Interaction of the Salmonella-containing vacuole with the endocytic recycling system.
    J Biol Chem. 280: 24634-41.
  12. Zuo, J. et al. (2008) The DNase of gammaherpesviruses impairs recognition by virus-specific CD8+ T cells through an additional host shutoff function.
    J Virol. 82: 2385-93.
  13. Shi, J. et al. (2008) Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
    Blood. 111: 1309-17.
  14. Watling, D. et al. (2008) Multiple kinases in the interferon-gamma response.
    Proc Natl Acad Sci U S A. 105: 6051-6.
  15. Grotzke, J.E. et al. (2009) The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle.
    PLoS Pathog. 5: e1000374.
  16. Koch, N. et al. (2009) IL-10 protects monocytes and macrophages from complement-mediated lysis.
    J Leukoc Biol. 86: 155-66.
  17. Spentzou, A. et al. (2010) Viral inhibition assay: a CD8 T cell neutralization assay for use in clinical trials of HIV-1 vaccine candidates.
    J Infect Dis. 201: 720-9.
  18. Vitadello, M. et al. (2010) Myofiber stress-response in myositis: parallel investigations on patients and experimental animal models of muscle regeneration and systemic inflammation.
    Arthritis Res Ther. 12: R52.
  19. Hinrichs, J. et al. (2010) The nature of peptides presented by an HLA class I low expression allele.
    Haematologica. 95: 1373-80.
  20. Fujita, Y. et al. (2010) Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice.
    Proc Natl Acad Sci U S A. 107: 14345-50.
  21. Narita, M. et al. (2010) WT1 peptide vaccination in combination with imatinib therapy for a patient with CML in the chronic phase.
    Int J Med Sci. 7: 72-81.
  22. Zuo, J. et al. (2011) The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting major histocompatibility complex class I molecules trafficking on both the exocytic and endocytic pathways.
    J Virol. 85: 1604-14.
  23. Lask, A. et al. (2011) TCR-Independent Killing of B Cell Malignancies by Anti-Third-Party CTLs: The Critical Role of MHC-CD8 Engagement.
    J Immunol. 187: 2006-14.
  24. Jones, D.C. et al. (2011) HLA Class I Allelic Sequence and Conformation Regulate Leukocyte Ig-Like Receptor Binding.
    J Immunol. 186: 2990-7.
  25. Enose-Akahata, Y. et al. (2012) Minocycline modulates antigen-specific CTL activity through inactivation of mononuclear phagocytes in patients with HTLV-I associated neurologic disease.
    Retrovirology. 9: 16.
  26. Badrinath, S. et al. (2012) Position 156 influences the peptide repertoire and tapasin dependency of human leukocyte antigen B*44 allotypes.
    Haematologica. 97: 98-106.
  27. Tannetta, D.S. et al. (2013) Characterisation of syncytiotrophoblast vesicles in normal pregnancy and pre-eclampsia: expression of Flt-1 and endoglin.
    PLoS One. 8 (2): e56754.
  28. Dragovic, R.A. et al. (2015) Isolation of syncytiotrophoblast microvesicles and exosomes and their characterisation by multicolour flow cytometry and fluorescence Nanoparticle Tracking Analysis.
    Methods. 87: 64-74.
  29. Tischer, S. et al. (2016) Discovery of immunodominant T-cell epitopes reveals penton protein as a second immunodominant target in human adenovirus infection.
    J Transl Med. 14 (1): 286.
  30. Praest, P. et al. (2019) A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway.
    Methods Mol Biol. 1988: 187-98.
  31. Juan, C.H. et al. (2020) In Vitro Differentiation of Human Placenta-Derived Multipotent Cells into Schwann-Like Cells.
    Biomolecules. 10 (12): 1657.
  32. Tupova, L. et al. (2020) Interplay of drug transporters P-glycoprotein (MDR1), MRP1, OATP1A2 and OATP1B3 in passage of maraviroc across human placenta.
    Biomed Pharmacother. 129: 110506.
  33. Nguyen, J. et al. (2021) Quantitative contributions of TNF receptor superfamily members to CD8+ T-cell responses.
    Mol Syst Biol. 17 (11): e10560.
  34. Xia, P. et al. (2022) NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis.
    BMC Immunol. 23 (1): 39.
  35. Cacciola, R. et al. (2022) Impact of Anti-Endothelial Cell Antibodies (AECAs) in Patients with Polycythemia Vera and Thrombosis
    Diagnostics. 12 (5): 1077.
  36. Korbonits, L. et al. (2022) Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome.
    Metabolites.12 (10): 924.
  37. Lyssy, F. et al. (2023) Platelet-derived factors dysregulate placental sphingosine-1-phosphate receptor 2 in human trophoblasts.
    Reprod Biomed Online. 47 (2): 103215.
  38. Forstner, D. et al. (2023) CD39 abrogates platelet-derived factors induced IL-1β expression in the human placenta.
    Front Cell Dev Biol. 11: 1183793.
  39. Zheng, S. et al. (2022) The SARS-CoV-2 accessory factor ORF7a downregulates MHC class I surface expression.
    bioRχiv 30 May [Epub ahead of print. Preprint]
  40. Fielding, C.A. et al. (2022) SARS-CoV-2 host-shutoff impacts innate NK cell functions, but antibody-dependent NK activity is strongly activated through non-spike antibodies.
    11: e74489.

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MCA81PB

1611

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