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HLA A2 antibody | BB7.2

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Anti Human HLA A2 Antibody, clone BB7.2 thumbnail image 1 Anti Human HLA A2 Antibody, clone BB7.2 thumbnail image 2 Anti Human HLA A2 Antibody, clone BB7.2 thumbnail image 3
Anti Human HLA A2 Antibody, clone BB7.2 gallery image 1
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Amethyst Orange conjugated Mouse IgG2b isotype control (MCA691AMO). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Amethyst Orange conjugated Mouse anti Human HLA-A2 (MCA2090AMO). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human HLA A2 Antibody, clone BB7.2 gallery image 2
Published customer image:
AlexaFluor®A647 conjugated Mouse anti HLA-2 antibody, clone BB7.2 (MCA2090A647)used to assess HLA-2 expression on SupT1 cells by flow cytometry,
Image caption:
Antigen-specific elimination of target cells by sTCR-Saporin as measured by flow cytometry.
(a) SupT1 cells expressing the indicated SCT were mixed at a 1:1 ratio with HLA-A2neg SupT1 cells and co-cultured for 3 days in the presence of relevant or irrelevant sTCR conjugated to Saporin, as indicated. At end of culture, cells were stained with anti-HLA-A2 AF647 to determine the frequencies of HLA-A2 positive and negative target cells among FSChi,SSChi events using flow cytometry. (b) Assay was performed as described in (a) and the percentage of SupT1 cells expressing indicated SCT that was eliminated by addition of sTCR is shown, calculated as described in materials and methods. Error bars indicate SEM of n = 3. (c) HLA-A2pos SupT1 cells mixed at a ratio of 1:1 with HLA-A2neg SupT1 cells were incubated overnight with indicated concentrations of peptide, washed, and indicated sTCR-Sap was added. SupT1 cells expressing SCT were used as positive controls (disconnected symbols). Culture and analysis to determine the percentage of eliminated HLA-A2pos targets was performed as described in (a,b). Results shown are representative of two experiments performed, and error bars represent SD of duplicates.

From: Walseng E, Wälchli S, Fallang L-E, Yang W, Vefferstad A, Areffard A, et al. (2015)
Soluble T-Cell Receptors Produced in Human Cells for Targeted Delivery.
PLoS ONE 10(4): e0119559.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human HLA A2 Antibody, clone BB7.2 gallery image 3
Published customer image:
AlexaFluor®A647 conjugated Mouse anti HLA-2 antibody, clone BB7.2 (MCA2090A647)used to assess HLA-2 expression on SupT1 cells by flow cytometry,
Image caption:
SupT1 cells, which are HLA-A2 negative, were transduced withSCT-M1, SCT-CD20 or SCT-EBV constructs, respectively. Cells staining positively with anti-HLA-A2 were sorted, expanded and stained with anti-HLA-A2 antibodies conjugated to Alexa Fluor 647 (anti-HLA-A2-AF647), as shown. Untransduced SupT1 cells (filled grey) were used as a negative control.

From: Walseng E, Wälchli S, Fallang L-E, Yang W, Vefferstad A, Areffard A, et al. (2015)
Soluble T-Cell Receptors Produced in Human Cells for Targeted Delivery.
PLoS ONE 10(4): e0119559.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human HLA A2:RPE

Product Type
Monoclonal Antibody
Clone
BB7.2
Isotype
IgG2b
Specificity
HLA A2

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Product Code Applications Pack Size List Price Your Price Qty
MCA2090PE
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Mouse anti Human HLA A2 antibody, clone BB7.2 recognizes the human HLA-A2 histocompatability antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands.

Mouse anti Human HLA A2 antibody, clone BB7.2 may be used for the flow cytometric detection of HLA-A2 expression and has also been used for immunoaffinity purification of HLA-A2 molecules. Functionally Mouse anti Human HLA A2 antibody, clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity.

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Papain solubilized HLA-A2
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.
DO NOT FREEZE.
This product is photosensitive and should be protected from light.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2b Negative Control:RPE MCA691PE F 100 Tests
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List Price Your Price
Description Mouse IgG2b Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

References for HLA A2 antibody

  1. Parham, P. & Brodsky, F.M. (1981) Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.
    Hum Immunol. 3 (4): 277-99.
  2. Hogan, K.T. & Brown, S.L. (1992) Localization and characterization of serologic epitopes on HLA-A2.
    Hum Immunol. 33 (3): 185-92.
  3. Drukker, M. et al. (2006) Human embryonic stem cells and their differentiated derivatives are less susceptible to immune rejection than adult cells.
    Stem Cells. 24 (2): 221-9.
  4. Pantenburg, B. et al. (2010) Human CD8(+) T cells clear Cryptosporidium parvum from infected intestinal epithelial cells.
    Am J Trop Med Hyg. 82:600-7.
  5. Duncan, L.M. et al. (2010) Stabilization of an E3 ligase-E2-ubiquitin complex increases cell surface MHC class I expression.
    J Immunol. 184: 6978-85.
  6. Wang, B. et al. (2004) Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein.
    Blood. 104: 200-6.
  7. Wooldridge, L. et al. (2007) Enhanced immunogenicity of CTL antigens through mutation of the CD8 binding MHC class I invariant region.
    Eur J Immunol. 37: 1323-33.
  8. Wooldridge, L. et al. (2010) MHC class I molecules with Superenhanced CD8 binding properties bypass the requirement for cognate TCR recognition and nonspecifically activate CTLs.
    J Immunol. 184: 3357-66.
    9. View The Latest Product References
  9. Popovic, J. et al. (2011) The only proposed T-cell epitope derived from the TEL-AML1 translocation is not naturally processed.
    Blood. 118 (4): 946-54.
  10. Schmidt, J. et al. (2011) Reversible Major Histocompatibility Complex I-Peptide Multimers Containing Ni2+-Nitrilotriacetic Acid Peptides and Histidine Tags Improve Analysis and Sorting of CD8+ T Cells.
    J Biol Chem. 286: 41723-35.
  11. Brooks, S.E. et al. (2015) Application of the pMHC Array to Characterise Tumour Antigen Specific T Cell Populations in Leukaemia Patients at Disease Diagnosis.
    PLoS One. 10 (10): e0140483.
  12. Rothe, K. et al. (2016) Latent Cytomegalovirus Infection in Rheumatoid Arthritis and Increased Frequencies of Cytolytic LIR-1+CD8+ T Cells.
    Arthritis Rheumatol. 68 (2): 337-46.
  13. De Angelis Rigotti, F. et al. (2017) MARCH9-mediated ubiquitination regulates MHC I export from the TGN.
    Immunol Cell Biol. 95 (9): 753-64.
  14. Kim, J.Y. et al. (2017) Detection of Donor-Derived Microparticles in the Peripheral Blood of a Hand Transplant Recipient During Rejection.
    Transplant Direct. 3 (3): e131.
  15. Dockree, T. et al. (2017) CD8+ T-cell specificity is compromised at a defined MHCI/CD8 affinity threshold.
    Immunol Cell Biol. 95 (1): 68-76.
  16. Sun, W. et al. (2018) A modified HLA-A*0201-restricted CTL epitope from human oncoprotein (hPEBP4) induces more efficient antitumor responses.
    Cell Mol Immunol. 15 (8): 768-81.
  17. Obenaus, M. et al. (2015) Identification of human T-cell receptors with optimal affinity to cancer antigens using antigen-negative humanized mice.
    Nat Biotechnol. 33 (4): 402-7.
  18. Walseng, E. et al. (2015) Soluble T-cell receptors produced in human cells for targeted delivery.
    PLoS One. 10 (4): e0119559.

Flow Cytometry

Functional Assays

RRID
AB_324027
UniProt
P01892
Entrez Gene
HLA-A

MCA2090PE

INN1801

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