Rat anti Human histone H4 antibody, clone 2B8
recognises histone H4 when it is dimethylated at K20. It does not bind to non-methylated H4. K20 is the only site on histone H4 methylated in vivo.
Rat anti Human histone H4 antibody, clone 2B8 has been reported for use in chromatin immunoprecipitation assay, see Beisel et al. 2002
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
CenH3-containing nucleosome assembly at centromere
negative regulation of megakaryocyte differentiation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Histone H4 Dimethyl K20 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for Histone H4 Dimethyl K20 antibody
Beisel, C. et al. (2002) Histone methylation by the Drosophila epigenetic transcriptional regulator Ash1.
Nature 419: 857-862. ?