Histone Deacetylase 4 antibody
Mouse anti Histone Deacetylase 4
Histone deacetylase 4 antibody recognizes histone deacetylase 4, also known as HDAC4 or histone deacetylase A. This antibody has been extensively validated for western blotting using whole cell lysates.
Histone Deacetylase 4 Antibody with Control Lysate
Histone deacetylase 4 antibody recognizes histone deacetylase 4, also known as HDAC4 or histone deacetylase A. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
---|---|---|---|---|---|---|
VMA00289 | WB | 100 µl | ||||
VMA00289KT | WB | 2 Westerns | ||||
With Lysate | 400µg |
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Encoded by the HDAC4 gene, histone deacetylase 4 belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3 (provided by RefSeq, Jul 2008).
Mouse anti Human histone deacetylase 4 antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti histone deacetylase 4 detects a band of approximately 140 kDa in Jurkat cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified recombinant fragment of human histone deacetylase 4 expressed in E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P56524 Related reagents
- Entrez Gene
- HDAC4 Related reagents
- GO Terms
- GO:0000118 histone deacetylase complex
- GO:0004407 histone deacetylase activity
- GO:0006338 chromatin remodeling
- GO:0006954 inflammatory response
- GO:0007399 nervous system development
- GO:0008270 zinc ion binding
- GO:0008284 positive regulation of cell proliferation
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010832 negative regulation of myotube differentiation
- GO:0014894 response to denervation involved in regulation of muscle adaptation
- GO:0014898 cardiac muscle hypertrophy in response to stress
- GO:0016563 transcription activator activity
- GO:0016566 specific transcriptional repressor activity
- GO:0017053 transcriptional repressor complex
- GO:0030183 B cell differentiation
- GO:0030955 potassium ion binding
- GO:0033235 positive regulation of protein sumoylation
- GO:0033613 activating transcription factor binding
- GO:0034983 peptidyl-lysine deacetylation
- GO:0042826 histone deacetylase binding
- GO:0043393 regulation of protein binding
- GO:0043433 negative regulation of transcription factor activity
- GO:0045820 negative regulation of glycolysis
- GO:0051091 positive regulation of transcription factor activity
- GO:0070491 repressing transcription factor binding
- GO:0070555 response to interleukin-1
- GO:0070932 histone H3 deacetylation
- GO:0070933 histone H4 deacetylation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Histone Deacetylase 4 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1/1000 | ||
Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Secondary Antibodies Available
Description | Product Code | Pack Size | Applications | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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