Histone Deacetylase 4 antibody

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Mouse anti Histone Deacetylase 4

Histone deacetylase 4 antibody recognizes histone deacetylase 4, also known as HDAC4 or histone deacetylase A. This antibody has been extensively validated for western blotting using whole cell lysates.

Histone Deacetylase 4 Antibody with Control Lysate

Histone deacetylase 4 antibody recognizes histone deacetylase 4, also known as HDAC4 or histone deacetylase A. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00289 WB 100 µl
VMA00289KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human histone deacetylase 4 antibody recognizes the histone deacetylase 4 (HDAC4), also known as histone deacetylase A.

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Encoded by the HDAC4 gene, histone deacetylase 4 belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3 (provided by RefSeq, Jul 2008).

Mouse anti Human histone deacetylase 4 antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti histone deacetylase 4 detects a band of approximately 140 kDa in Jurkat cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human histone deacetylase 4 expressed in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P56524 Related reagents
Entrez Gene
HDAC4 Related reagents
GO Terms
GO:0000118 histone deacetylase complex
GO:0004407 histone deacetylase activity
GO:0006338 chromatin remodeling
GO:0006954 inflammatory response
GO:0007399 nervous system development
GO:0008270 zinc ion binding
GO:0008284 positive regulation of cell proliferation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010832 negative regulation of myotube differentiation
GO:0014894 response to denervation involved in regulation of muscle adaptation
GO:0014898 cardiac muscle hypertrophy in response to stress
GO:0016563 transcription activator activity
GO:0016566 specific transcriptional repressor activity
GO:0017053 transcriptional repressor complex
GO:0030183 B cell differentiation
GO:0030955 potassium ion binding
GO:0033235 positive regulation of protein sumoylation
GO:0033613 activating transcription factor binding
GO:0034983 peptidyl-lysine deacetylation
GO:0042826 histone deacetylase binding
GO:0043393 regulation of protein binding
GO:0043433 negative regulation of transcription factor activity
GO:0045820 negative regulation of glycolysis
GO:0051091 positive regulation of transcription factor activity
GO:0070491 repressing transcription factor binding
GO:0070555 response to interleukin-1
GO:0070932 histone H3 deacetylation
GO:0070933 histone H4 deacetylation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Histone Deacetylase 4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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