Histone Deacetylase 3 antibody | E04/3H10

100% Secure


Mouse anti Histone Deacetylase 3

Product Type
PrecisionAb Monoclonal
Clone
E04/3H10
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00733
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human histone deacetylase 3 antibody recognizes histone deacetylase 3 (HDAC3), also known as SMAP45.

HDAC3 is a ubiquitously-expressed histone deacetylase, mostly located in the nucleus within transcriptional coregulator complexes (Song et al. 2019). By inhibiting the activity of multiple proteins, HDAC3 plays important roles in physiological homeostasis in many tissues (Kwon et al. 2018). In addition to its enzymatic role in catalysis of deacetylation reactions, HDAC3 can regulate chromatin accessibility and corepressor complex dynamics by acting as a chaperone or a scaffold anchorage protein, instead of an enzyme (Song et al. 2019). HDAC3 contributes to inflammation, cancer, and degenerative neurological diseases, and inhibitors of HDAC3 have been suggested as potential treatments (Cao et al. 2018). HDAC3 overexpression is associated with poor prognosis in a range of cancer types, however, the role of HDAC3 in cancer is tissue-specific. HDAC3 can also regulate responses to anticancer drugs (Kwon et al. 2018).

Product Details

Target Species
Human
Western Blotting
Mouse anti histone deacetylase 3 detects a band of approximately 47 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli--derived recombinant protein of amino acids 1-428 of human histone deacetylase 3
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
O15379
Entrez Gene
HDAC3
GO Terms
GO:0000118 histone deacetylase complex
GO:0003714 transcription corepressor activity
GO:0004407 histone deacetylase activity
GO:0005737 cytoplasm
GO:0005876 spindle microtubule
GO:0006916 anti-apoptosis
GO:0008134 transcription factor binding
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0016564 transcription repressor activity
GO:0017053 transcriptional repressor complex
GO:0042826 histone deacetylase binding
GO:0044255 cellular lipid metabolic process
GO:0045786 negative regulation of cell cycle
GO:0046329 negative regulation of JNK cascade
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051225 spindle assembly
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Histone Deacetylase 3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml