HIST2H2BE antibody | 10G12-2C12

Mouse anti HIST2H2BE

Product Type
PrecisionAb Monoclonal
Clone
10G12-2C12
Isotype
IgG1 Kappa
Format
Purified
Specificity
HIST2H2BE

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VMA00873
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Mouse anti HIST2H2BE antibody recognizes histone H2B type 2-E, also known as H2BC21 and H2BFQ.

HIST2H2BE is a member of the histone family and a component of the nucleosome, responsible for the chromosomal fibre nucleosome structure (Li et al. 2019). The nucleosome creates a structure to the DNA and acts as a signaling hub for chromatin enzymes and post-translational modifications, as well as being able to assemble into higher-order structures allowing genome compaction (McGinty and Tan 2015). Histones are proteins with a positive charge, allowing them to associate with negatively-charged DNA and provide nucleosome structure. The nucleosome core particle is composed of two copies of the following histones: H2A, H2B, H3 and H4 with DNA wrapped around it (Mariño-Ramírez et al. 2007).

Mouse anti HIST2H2BE antibody recognizes epitope sequence corresponding to amino acids 103 to 109 of HIST2H2BE.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti HIST2H2BE detects a band of approximately 15 kDa
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to HIST2HBE amino acids 96 to 126
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20oC, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt
Q16778
Entrez Gene
HIST2H2BE
GO Terms
GO:0005515 protein binding
GO:0000786 nucleosome
GO:0003677 DNA binding
GO:0006334 nucleosome assembly
GO:0042742 defense response to bacterium
GO:0005634 nucleus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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