HIST2H2BE antibody | 10G12-2C12
Mouse anti HIST2H2BE antibody recognizes histone H2B type 2-E, also known as H2BC21 and H2BFQ.
HIST2H2BE is a member of the histone family and a component of the nucleosome, responsible for the chromosomal fibre nucleosome structure (Li et al. 2019). The nucleosome creates a structure to the DNA and acts as a signaling hub for chromatin enzymes and post-translational modifications, as well as being able to assemble into higher-order structures allowing genome compaction (McGinty and Tan 2015). Histones are proteins with a positive charge, allowing them to associate with negatively-charged DNA and provide nucleosome structure. The nucleosome core particle is composed of two copies of the following histones: H2A, H2B, H3 and H4 with DNA wrapped around it (Mariño-Ramírez et al. 2007).
Mouse anti HIST2H2BE antibody recognizes epitope sequence corresponding to amino acids 103 to 109 of HIST2H2BE.
HIST2H2BE is a member of the histone family and a component of the nucleosome, responsible for the chromosomal fibre nucleosome structure (Li et al. 2019). The nucleosome creates a structure to the DNA and acts as a signaling hub for chromatin enzymes and post-translational modifications, as well as being able to assemble into higher-order structures allowing genome compaction (McGinty and Tan 2015). Histones are proteins with a positive charge, allowing them to associate with negatively-charged DNA and provide nucleosome structure. The nucleosome core particle is composed of two copies of the following histones: H2A, H2B, H3 and H4 with DNA wrapped around it (Mariño-Ramírez et al. 2007).
Mouse anti HIST2H2BE antibody recognizes epitope sequence corresponding to amino acids 103 to 109 of HIST2H2BE.
Product Details
- Target Species
- Human
- Western Blotting
- Anti HIST2H2BE detects a band of approximately 15 kDa
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to HIST2HBE amino acids 96 to 126
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q16778
- Entrez Gene
- HIST2H2BE
- GO Terms
- GO:0005515 protein binding
- GO:0000786 nucleosome
- GO:0003677 DNA binding
- GO:0006334 nucleosome assembly
- GO:0042742 defense response to bacterium
- GO:0005634 nucleus
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of HIST2H2BE antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
