High Mobility Group Protein HMGI-C antibody
Mouse anti High Mobility Group Protein HMGI-C
Mouse anti-human high mobility group protein HMGI-C antibody recognizes high mobility group protein HMGI-C, also known as high mobility group AT-hook protein 2.
High Mobility Group Protein HMGI-C Antibody with Control Lysate
Mouse anti-human high mobility group protein HMGI-C antibody recognizes high mobility group protein HMGI-C, also known as high mobility group AT-hook protein 2.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00420 | WB | 100 µl | ||||
| VMA00420KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human high mobility group protein HMGI-C antibody recognizes high-mobility group protein HMGI-C also known as high mobility group AT-hook 2 and HMGA2.
The HMGA2 gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhanceosome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of HMGA2 that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that HMGA2 is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized (provided by RefSeq, Jul 2008).
Mouse anti Human high mobility group protein HMGI-C antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The HMGA2 gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhanceosome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of HMGA2 that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that HMGA2 is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized (provided by RefSeq, Jul 2008).
Mouse anti Human high mobility group protein HMGI-C antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti high mobility group protein HMGI-C detects a band of approximately 18 kDa in HepG2 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E.coli-derived recombinant protein corresponding to aa 1-109 of human high mobility group protein HMGI-C
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HepG2 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P52926 Related reagents
- Entrez Gene
- HMGA2 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0000785 chromatin
- GO:0003680 AT DNA binding
- GO:0005634 nucleus
- GO:0006325 chromatin organization
- GO:0006350 transcription
- GO:0006355 regulation of transcription, DNA-dependent
- GO:0007067 mitosis
- GO:0007275 multicellular organismal development
- GO:0040008 regulation of growth
- GO:0051301 cell division
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of High Mobility Group Protein HMGI-C antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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