HIF1AN antibody

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Mouse anti HIF1AN

Mouse anti-human HIF1AN antibody recognizes hypoxia-inducible factor 1-alpha inhibitor, also known as FIH-1, factor inhibiting HIF-1, hypoxia-inducible factor asparagine hydroxylase or peptide-aspartate beta-dioxygenase.

HIF1AN Antibody with Control Lysate

Mouse anti-human HIF1AN antibody recognizes hypoxia-inducible factor 1-alpha inhibitor, also known as FIH-1, factor inhibiting HIF-1, hypoxia-inducible factor asparagine hydroxylase or peptide-aspartate beta-dioxygenase.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00490 WB 100 µl
VMA00490KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human HIF1AN antibody recognizes HIF1AN also known as hypoxia-inducible factor 1-alpha inhibitor, factor inhibiting HIF-1, hypoxia-inducible factor asparagine hydroxylase or peptide-aspartate beta-dioxygenase.

Mouse anti Human HIF1AN antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Full length human HIF1AN expressed in E. Coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9NWT6 Related reagents
Entrez Gene
HIF1AN Related reagents
GO Terms
GO:0005515 protein binding
GO:0004597 peptide-aspartate beta-dioxygenase activity
GO:0046872 metal ion binding
GO:0005634 nucleus
GO:0055114 oxidation-reduction process
GO:0006350 transcription
GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0045449 regulation of transcription
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of HIF1AN antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti HIF1AN detects a band of approximately 42 kDa in HEK293 cell lysate
Western Blotting
Anti HIF1AN detects a band of approximately 42 kDa in HEK293 cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB