HIF1 Alpha antibody | ESEE122

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Mouse anti Human HIF1 Alpha:Biotin

Mouse anti Human HIF1 Alpha

Product Type
Monoclonal Antibody
Clone
ESEE122
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5695B E P* WB 0.1 mg
MCA5695GA E P* WB 0.1 mg
MCA5695 E P* WB 0.2 mg
Mouse anti Human HIF1 alpha antibody, clone ESEE122 recognizes human HIF1α, also known as Hypoxia-inducible factor 1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1 or PAS domain-containing protein 8. HIF1α is a widely expressed bHLH and PAS domain containing transcription factor, a critical regulatory protein in the host response to hypoxia.

HIF1α interacts with the HIF1β/ARNT subunit to form the highly-conserved heterodimeric HIF-1 transcriptional complex. During hypoxic conditions, HIF1α translocated from a normoxic cytoplasmic location to the nucleus and activates transcription of several genes including erythropoietin, VEGF and glucose transporters, encoding proteins involved in oxygen delivery and metabolic adaptation to hypoxia. Under normal oxygen conditions, HIF1α is targeted by HIF prolyl-hydroxylase, followed by rapid proteolytic degradation. Overexpression of HIF1α occurs in many common human cancers, including pancreatic, bladder and renal carcinomas (Semenza 2003).

Mouse anti Human HIF1 alpha antibody, clone ESEE122 has been shown to be suitable for the staining of snap-frozen cell pellets (Talks. et al. 2000).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant human HIF1 alpha
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS-1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from the date of despatch.
Shelf Life
18 months from the date of despatch.

More Information

UniProt
Q16665 Related reagents
Entrez Gene
HIF1A Related reagents
GO Terms
GO:0001666 response to hypoxia
GO:0001837 epithelial to mesenchymal transition
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002248 connective tissue replacement involved in inflammatory response wound healing
GO:0003677 DNA binding
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0004871 signal transducer activity
GO:0005737 cytoplasm
GO:0005667 transcription factor complex
GO:0005730 nucleolus
GO:0008134 transcription factor binding
GO:0051879 Hsp90 protein binding
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010634 positive regulation of epithelial cell migration
GO:0046886 positive regulation of hormone biosynthetic process
GO:0030528 transcription regulator activity
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0032364 oxygen homeostasis
GO:0032722 positive regulation of chemokine production
GO:0032909 regulation of transforming growth factor-beta2 production
GO:0032963 collagen metabolic process
GO:0035035 histone acetyltransferase binding
GO:0042789 mRNA transcription from RNA polymerase II promoter
GO:0043193 positive regulation of gene-specific transcription
GO:0043619 regulation of transcription from RNA polymerase II promoter in response to oxidative stress
GO:0045648 positive regulation of erythrocyte differentiation
GO:0045766 positive regulation of angiogenesis
GO:0045821 positive regulation of glycolysis
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046982 protein heterodimerization activity
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:0051541 elastin metabolic process
Regulatory
For research purposes only

Applications of HIF1 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1
Western Blotting
ELISA
Immunohistology - Paraffin 1 1/100 1/500
Western Blotting
  1. 1 Special conditions apply - see [*]
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    EDTA pH9.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
*This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase, or antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA pH9.0 is recommended for this purpose.
Histology Positive Control Tissue
Human tonsil
Histology Positive Control Tissue
Human tonsil
Western Blotting
MCA5695B detects a band of approximately 135kDa in 0.1% hypoxia HeLa cell lysates(1).
Western Blotting
MCA5695 detects a band of approximately 135kDa in 0.1% hypoxia HeLa cell lysates(1).

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for HIF1 Alpha antibody

  1. Talks, K.L. et al. (2000) The expression and distribution of the hypoxia-inducible factors HIF-1alpha and HIF-2alpha in normal human tissues, cancers, and tumor-associated macrophages.
    Am J Pathol. 157 (2): 411-21.

Further Reading

  1. Semenza, G.L. (2000) HIF-1 and human disease: one highly involved factor.
    Genes Dev. 14 (16): 1983-91.