Hexokinase 1 antibody | 4D7
A: hexokinase specificity against recombinant hexokinase proteins
B: hexokinase expression in human cell lines and mouse brain
Mouse anti Human hexokinase 1, clone 4D7 recognizes human hexokinase 1, a 102 kDa major allosteric enzyme of the glycolytic pathway, which catalyses the phosphorylation of glucose to form glucose-6-phosphate (Glc-6-P).
Hexokinase 1 is the first of four distinct mammalian hexokinase isozymes (hexokinase type 1 to 4), expressed in the outer membrane of mitochondria and is regulated (inhibited) by its product Glc-6-P. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia (Newman et al. 1980).
Mouse anti hexokinase 1, clone 4D7 recognizes an epitope common to all isoforms of human hexokinase.
Hexokinase 1 is the first of four distinct mammalian hexokinase isozymes (hexokinase type 1 to 4), expressed in the outer membrane of mitochondria and is regulated (inhibited) by its product Glc-6-P. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia (Newman et al. 1980).
Mouse anti hexokinase 1, clone 4D7 recognizes an epitope common to all isoforms of human hexokinase.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 10% Glycerol
- Immunogen
- Recombinant human hexokinase 1 (amino acids 1-917) purified from Escherichia coli.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P19367 Related reagents
- Entrez Gene
- HK1 Related reagents
- GO Terms
- GO:0004340 glucokinase activity
- GO:0005829 cytosol
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0005741 mitochondrial outer membrane
- GO:0006096 glycolysis
- GO:0015758 glucose transport
- GO:0055085 transmembrane transport
- Regulatory
- For research purposes only
Applications of Hexokinase 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting | 1/1000 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- MCA2698 detects a band of approximately 102kDa in HepG2 cell lysates.
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Secondary Antibodies Available
Product Specific References
Further Reading
-
Wilson, J.E. (2003) Isozymes of mammalian hexokinase: structure, subcellular localization and metabolic function.
J Exp Biol. 206 (Pt 12): 2049-57.
