Heparanase-1 antibody | HP3/17
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Mouse anti Human Heparanase-1
- Product Type
- Monoclonal Antibody
- Clone
- HP3/17
- Isotype
- IgG2b
- Specificity
- Heparanase-1
Antibody Quality and 
Unrivaled 
| Mouse anti Human Heparanase-1 antibody, clone HP3/17 recognizes heparanase-1, also know as HPA1, an endo-beta-D-glucuronidase that degrades heparan sulfate proteoglycans (HSPGs) in the extracellular matrix through cleavage of the heparan sulfate side chains. Heparanase-1 is a heterodimer that is produced as a 65 kDa latent precursor, consisting of an 8 kDa subunit, a 50 kDa subunit and a linker peptide. Activation of Heparanase-1 occurs through cleavage of the linker peptide, releasing the 8 kDa subunit from the active 50 kDa subunit. Mouse anti Heparanase-1 has been reported to recognize heparanase-1 in xenopus by western blotting (Bertolesi et al. 2008) |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Xenopus - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Polypeptide from the 50 kDa subunit of heparanase.
- Purity
- 98% by SDS PAGE
- Approx. Protein Concentrations
- Pack Size: 50 µg, 0.15 mgIgG concentration 4.0 mg/mlPack Size: 0.1 mgIgG concentration 3.57 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin |  |
1/40 | 1/40 |
| Western Blotting | |
1/4000 | 1/4000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- OBT1975 detects bands of approximately 50kDa (corresponding to the subunit containing the active site) and 65kDa (the precursor of heparanase-1) when purified recombinant human heparanase samples are used.
References for Heparanase-1 antibody
-
Stuart, H.M. et al. (2015) Urinary Tract Effects of HPSE2 Mutations.
J Am Soc Nephrol. 26 (4): 797-804.
- RRID
- AB_2120722
- UniProt
- Q9Y251
- Entrez Gene
- HPSE
- GO Terms
- GO:0006029 proteoglycan metabolic process
- GO:0004566 beta-glucuronidase activity
- GO:0005576 extracellular region
- GO:0005765 lysosomal membrane
- GO:0005975 carbohydrate metabolic process
- GO:0043169 cation binding
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