Heparanase-1 antibody | HP3/17

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Mouse anti Human Heparanase-1

Product Type
Monoclonal Antibody
Clone
HP3/17
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
OBT1975 P WB 50 µg
OBT1975G P WB 0.1 mg
OBT1975X P WB 0.15 mg
Mouse anti Human Heparanase-1 antibody, clone HP3/17 recognizes heparanase-1, also know as HPA1, an endo-beta-D-glucuronidase that degrades heparan sulfate proteoglycans (HSPGs) in the extracellular matrix through cleavage of the heparan sulfate side chains.

Heparanase-1 is a heterodimer that is produced as a 65 kDa latent precursor, consisting of an 8 kDa subunit, a 50 kDa subunit and a linker peptide. Activation of Heparanase-1 occurs through cleavage of the linker peptide, releasing the 8 kDa subunit from the active 50 kDa subunit.

Mouse anti Heparanase-1 has been reported to recognize heparanase-1 in xenopus by western blotting (Bertolesi et al. 2008)

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Xenopus
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Polypeptide from the 50 kDa subunit of heparanase.
Purity
98% by SDS PAGE
Approx. Protein Concentrations
Pack Size: 50 µg, 0.15 mg
IgG concentration 4.0 mg/ml
Pack Size: 0.1 mg
IgG concentration 3.57 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9Y251 Related reagents
Entrez Gene
HPSE Related reagents
GO Terms
GO:0006029 proteoglycan metabolic process
GO:0004566 beta-glucuronidase activity
GO:0005576 extracellular region
GO:0005765 lysosomal membrane
GO:0005975 carbohydrate metabolic process
GO:0043169 cation binding
Regulatory
For research purposes only

Applications of Heparanase-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/40 1/40
Western Blotting 1/4000 1/4000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
OBT1975 detects bands of approximately 50kDa (corresponding to the subunit containing the active site) and 65kDa (the precursor of heparanase-1) when purified recombinant human heparanase samples are used.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Heparanase-1 antibody

  1. Stuart, H.M. et al. (2015) Urinary Tract Effects of HPSE2 Mutations.
    J Am Soc Nephrol. 26 (4): 797-804.