Hemoglobin Subunit zeta antibody | 3C4-1D5
Mouse anti Hemoglobin subunit zeta antibody, clone 3C4-1D5 recognizes the human embryonic hemoglobin zeta chain, also known as Zeta-globin, HBAZ or Hb-ζ. Hemoglobin zeta is a 141 amino acid ~14 kDa alpha-type hemoglobin chain present in early embryonic erythrocytes, forming part of the active tetrameric oxygen binding hemoglobin units Gower-1 (ζ2:γ2) Portland-1 (ζ2:ε2) and Portland-2 (ζ2:β2). Gower-2 hemoglobin does not contain any zeta components (α2:ε2) (Manning et al. 2007). Portland-2 hemoglobin containing 2ζ chains is associated with homozygous alpha thalassemia (Li et al. 2010).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant protein corresponding to aa 1-143 of human Hemoglobin subunit zeta.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- P02008 Related reagents
- Entrez Gene
- HBZ Related reagents
- GO Terms
- GO:0005344 oxygen transporter activity
- GO:0005833 hemoglobin complex
- GO:0019825 oxygen binding
- GO:0020037 heme binding
- For research purposes only
Applications of Hemoglobin Subunit zeta antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Western Blotting||0.1||10 ug/ml|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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