Mouse anti Hemoglobin subunit zeta antibody, clone 3C4-1D5
recognizes the human embryonic hemoglobin zeta chain, also known as Zeta-globin, HBAZ or Hb-ζ. Hemoglobin zeta is a 141 amino acid ~14 kDa alpha-type hemoglobin chain present in early embryonic erythrocytes, forming part of the active tetrameric oxygen binding hemoglobin units Gower-1 (ζ2:γ2) Portland-1 (ζ2:ε2) and Portland-2 (ζ2:β2). Gower-2 hemoglobin does not contain any zeta components (α2:ε2) (Manning et al. 2007
). Portland-2 hemoglobin containing 2ζ chains is associated with homozygous alpha thalassemia (Li et al. 2010
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant protein corresponding to aa 1-143 of human Hemoglobin subunit zeta.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
oxygen transporter activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Hemoglobin Subunit zeta antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
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