Hemoglobin antibody | AbD05988

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Human anti Human Hemoglobin

Product Type
Monoclonal Antibody
Clone
AbD05988
Isotype
HuCAL Fab monovalent
Product Code Applications Pack Size List Price Quantity
HCA042
Datasheet
E WB
MSDS
0.1 mg loader

Human anti Human hemoglobin antibody, clone AbD05988 recognizes human hemoglobin, a vital oxygen transporting protein that is the major protein constituent of red blood cells.

This antibody recognizes human hemoglobin in Western blot, binding to the 17 kDa sub-unit and multimers thereof. Analysis of binding has demonstrated an affinity of 9nM.

Product Details

Target Species
Human
Product Form
A monovalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Purified human hemoglobin
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
6 months from date of despatch

More Information

UniProt
P69905 Related reagents
P68871 Related reagents
Entrez Gene
HBB Related reagents
HBA2 Related reagents
GO Terms
GO:0022627 cytosolic small ribosomal subunit
GO:0005515 protein binding
GO:0005344 oxygen transporter activity
GO:0005833 hemoglobin complex
GO:0010942 positive regulation of cell death
GO:0019825 oxygen binding
GO:0020037 heme binding
GO:0042744 hydrogen peroxide catabolic process
GO:0051291 protein heterooligomerization
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Hemoglobin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/1000 1/2500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Human IgG F(ab')2:HRP 0500-0099 E WB 0.5 ml loader
Mouse anti c-Myc:HRP MCA2200P C E P WB* 0.1 mg loader
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader