Heat Shock Protein 90 Beta antibody

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Mouse anti Heat Shock Protein 90 Beta

Heat shock protein 90-beta antibody recognizes human heat shock protein 90-beta (HSP 90-beta), also known as heat shock 84 kDa or heat shock 90 kDa protein 1. This antibody has been extensively validated for western blotting using whole cell lysates.

Heat Shock Protein 90 Beta Antibody with Control Lysate

Heat shock protein 90-beta antibody recognizes human heat shock protein 90-beta (HSP 90-beta), also known as heat shock 84 kDa or heat shock 90 kDa protein 1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00082 WB 100 µl
VMA00082KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human heat shock protein 90 beta antibody recognizes heat shock protein 90 beta , also known as HSP 90, Heat shock 84 kDa, HSP 84, HSP84, 3D polymerase, 3Dpol Protein 3D, 3D.

Heat shock protein 90 beta a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. HSP90AB1 encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. (provided by RefSeq, Dec 2012).

Mouse anti Human heat shock protein 90 beta antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti heat shock protein 90 beta detects a band of approximately 90 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
10% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
10% Glycerol
Immunogen
Recombinant human Hsp 90 alpha
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P08238 Related reagents
Entrez Gene
HSP90AB1 Related reagents
GO Terms
GO:0051082 unfolded protein binding
GO:0006457 protein folding
GO:0006986 response to unfolded protein
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0007411 axon guidance
GO:0030235 nitric-oxide synthase regulator activity
GO:0030911 TPR domain binding
GO:0032435 negative regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0042470 melanosome
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
GO:0060338 regulation of type I interferon-mediated signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Heat Shock Protein 90 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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