Heat Shock Protein 90 Beta antibody
Mouse anti Heat Shock Protein 90 Beta
Heat shock protein 90-beta antibody recognizes human heat shock protein 90-beta (HSP 90-beta), also known as heat shock 84 kDa or heat shock 90 kDa protein 1. This antibody has been extensively validated for western blotting using whole cell lysates.
Heat Shock Protein 90 Beta Antibody with Control Lysate
Heat shock protein 90-beta antibody recognizes human heat shock protein 90-beta (HSP 90-beta), also known as heat shock 84 kDa or heat shock 90 kDa protein 1. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG2a
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00082 | WB | 100 µl | ||||
| VMA00082KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human heat shock protein 90 beta antibody recognizes heat shock protein 90 beta , also known as HSP 90, Heat shock 84 kDa, HSP 84, HSP84, 3D polymerase, 3Dpol Protein 3D, 3D.
Heat shock protein 90 beta a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. HSP90AB1 encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. (provided by RefSeq, Dec 2012).
Mouse anti Human heat shock protein 90 beta antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Heat shock protein 90 beta a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. HSP90AB1 encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. (provided by RefSeq, Dec 2012).
Mouse anti Human heat shock protein 90 beta antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti heat shock protein 90 beta detects a band of approximately 90 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
10% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
10% Glycerol - Immunogen
- Recombinant human Hsp 90 alpha
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P08238 Related reagents
- Entrez Gene
- HSP90AB1 Related reagents
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0006457 protein folding
- GO:0006986 response to unfolded protein
- GO:0005829 cytosol
- GO:0005524 ATP binding
- GO:0007411 axon guidance
- GO:0030235 nitric-oxide synthase regulator activity
- GO:0030911 TPR domain binding
- GO:0032435 negative regulation of proteasomal ubiquitin-dependent protein catabolic process
- GO:0042470 melanosome
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0060334 regulation of interferon-gamma-mediated signaling pathway
- GO:0060338 regulation of type I interferon-mediated signaling pathway
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Heat Shock Protein 90 Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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