Heat Shock Protein 90 Beta antibody

100% Secure

Mouse anti Heat Shock Protein 90 Beta

Heat shock protein 90-beta antibody recognizes human heat shock protein 90-beta (HSP 90-beta), also known as heat shock 84 kDa or heat shock 90 kDa protein 1. This antibody has been extensively validated for western blotting using whole cell lysates.

Heat Shock Protein 90 Beta Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG2a
Format: Purified

Product Code	Applications	Pack Size
VMA00082	WB	100 µl
VMA00082KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human heat shock protein 90 beta antibody recognizes heat shock protein 90 beta , also known as HSP 90, Heat shock 84 kDa, HSP 84, HSP84, 3D polymerase, 3Dpol Protein 3D, 3D.

Heat shock protein 90 beta a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. HSP90AB1 encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. (provided by RefSeq, Dec 2012).

Mouse anti Human heat shock protein 90 beta antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti heat shock protein 90 beta detects a band of approximately 90 kDa in Jurkat cell lysates.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
10% Glycerol

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
10% Glycerol

Immunogen

Recombinant human Hsp 90 alpha

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P08238 Related reagents
Entrez Gene: HSP90AB1 Related reagents
GO Terms: GO:0051082 unfolded protein binding; GO:0006457 protein folding; GO:0006986 response to unfolded protein; GO:0005829 cytosol; GO:0005524 ATP binding; GO:0007411 axon guidance; GO:0030235 nitric-oxide synthase regulator activity; GO:0030911 TPR domain binding; GO:0032435 negative regulation of proteasomal ubiquitin-dependent protein catabolic process; GO:0042470 melanosome; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0060334 regulation of interferon-gamma-mediated signaling pathway; GO:0060338 regulation of type I interferon-mediated signaling pathway
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Heat Shock Protein 90 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information

Online Payment Methods:

Invoice / Purchase Order
Credit Card (Maestro, VISA,MasterCard & VISA Electron)

View more products with Heat Shock Protein 90 Beta specificity