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Heat Shock Protein 90 antibody | S88

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Anti Heat Shock Protein 90 Antibody, clone S88 thumbnail image 1 Anti Heat Shock Protein 90 Antibody, clone S88 thumbnail image 2
Anti Heat Shock Protein 90 Antibody, clone S88 gallery image 1
Western blot analysis of HeLa cell lysate using mouse anti heat shock protein 90 antibody, clone S88 (MCA6018) which recognizes a band of approximately 90 kDa.
Anti Heat Shock Protein 90 Antibody, clone S88 gallery image 2
Western blot analysis of HeLa cell lysate using Mouse anti heat shock protein 90 antibody, clone S88 (MCA6018) which recognizes a band of approximately 90 kDa. HeLa cell lysate was run in lanes 2, 3 and 4. Lane 2 was probed with 2 μg/ml antibody and lane 3 was probed with 1 μg/ml antibody. STAR207P was used as the secondary antibody and the blot was visualized with the Bio-Rad ChemiDoc system. STAR207P was run in lane 4 without the primary antibody as a negative control. Bio-Rad Precision Plus molecular weight marker was run in lane 1.

Mouse anti Heat Shock Protein 90

Product Type
Monoclonal Antibody
Clone
S88
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6018 IP P WB 50 µg
Mouse anti heat shock protein 90 antibody, clone S88, recognizes the alpha and beta forms of heat shock protein 90. Heat shock protein 90 is a highly abundant protein and acts as a chaperone. It plays a crucial role in protecting cells against heat induced stress.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
Monkey Expected from Sequence
Hamster Expected from Sequence
Rabbit Expected from Sequence
Bovine Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.2% Thiomersal
0.1% Bovine Serum Albumin
50% Glycerol
Immunogen
Achlya ambisexualis heat shock protein 90
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P07900 Related reagents
Entrez Gene
HSP90AA1 Related reagents
GO Terms
GO:0051082 unfolded protein binding
GO:0005886 plasma membrane
GO:0000086 G2/M transition of mitotic cell cycle
GO:0006986 response to unfolded protein
GO:0042026 protein refolding
GO:0005829 cytosol
GO:0007165 signal transduction
GO:0005524 ATP binding
GO:0007411 axon guidance
GO:0030235 nitric-oxide synthase regulator activity
GO:0030911 TPR domain binding
GO:0034619 cellular chaperone-mediated protein complex assembly
GO:0042470 melanosome
GO:0042803 protein homodimerization activity
GO:0045040 protein import into mitochondrial outer membrane
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0046209 nitric oxide metabolic process
GO:0050999 regulation of nitric-oxide synthase activity
Regulatory
For research purposes only

Applications of Heat Shock Protein 90 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 10 20 ug/ml
Immunoprecipitation 4 8 ug/ml
Western Blotting 1 2 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Mouse anti heat shock protein 90 antibody (clone S88) recognizes a band of approximately 90 kDa in heat shocked HeLa cell lysate. The antibody detects both alpha and beta forms.
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