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Heat Shock Protein 90 Alpha antibody | OTI3B5

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Western Blot Validated
Anti Heat Shock Protein 90 Alpha Antibody, clone OTI3B5 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with heat shock protein 90 alpha antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to heat shock protein 90 alpha (molecular weight 90 kDa)

Mouse anti Heat Shock Protein 90 Alpha

Product Type
PrecisionAb Monoclonal
Clone
OTI3B5
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00689
Datasheet
WB
SDS
100 µl loader

Mouse anti Human heat shock protein 90 alpha antibody recognizes HSP90 alpha, also known as LPS-associated protein 2, LAP2, HSPAA1 and HSPN.

The protein encoded by HSP90AA1 is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for HSP90AA1 (provided by RefSeq, Jan 2012).

Mouse anti Human heat shock protein 90 alpha antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti heat shock protein 90 alpha detects a band of approximately 90 kDa in NIH/3T3 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen
HEK293-derived full length recombinant protein of human heat shock protein 90 alpha

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P07900
Entrez Gene
HSP90AA1
GO Terms
GO:0051082 unfolded protein binding
GO:0005886 plasma membrane
GO:0000086 G2/M transition of mitotic cell cycle
GO:0006986 response to unfolded protein
GO:0042026 protein refolding
GO:0005829 cytosol
GO:0007165 signal transduction
GO:0005524 ATP binding
GO:0007411 axon guidance
GO:0030235 nitric-oxide synthase regulator activity
GO:0030911 TPR domain binding
GO:0034619 cellular chaperone-mediated protein complex assembly
GO:0042470 melanosome
GO:0042803 protein homodimerization activity
GO:0045040 protein import into mitochondrial outer membrane
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0046209 nitric oxide metabolic process
GO:0050999 regulation of nitric-oxide synthase activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Heat Shock Protein 90 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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