Heat Shock Protein 90 Alpha antibody | 3B5
Mouse anti Heat Shock Protein 90 Alpha
Heat shock protein 90 alpha antibody recognizes HSP 90 alpha, also known as HSP90, LPS-associated protein 2, LAP2, HSPAA1, or HSPN.
Heat Shock Protein 90 Alpha Antibody with Control Lysate
Heat shock protein 90 alpha antibody recognizes HSP 90 alpha, also known as HSP90, LPS-associated protein 2, LAP2, HSPAA1, or HSPN.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- 3B5
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00689 | WB | 100 µl | ||||
| VMA00689KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human heat shock protein 90 alpha antibody recognizes HSP90 alpha, also known as LPS-associated protein 2, LAP2, HSPAA1 and HSPN.
The protein encoded by HSP90AA1 is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for HSP90AA1 (provided by RefSeq, Jan 2012).
Mouse anti Human heat shock protein 90 alpha antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by HSP90AA1 is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for HSP90AA1 (provided by RefSeq, Jan 2012).
Mouse anti Human heat shock protein 90 alpha antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti heat shock protein 90 alpha detects a band of approximately 90 kDa in NIH/3T3 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody affinity purified on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin - Immunogen
- HEK293-derived full length recombinant protein of human heat shock protein 90 alpha
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg NIH/3T3 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P07900 Related reagents
- Entrez Gene
- HSP90AA1 Related reagents
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0005886 plasma membrane
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0006986 response to unfolded protein
- GO:0042026 protein refolding
- GO:0005829 cytosol
- GO:0007165 signal transduction
- GO:0005524 ATP binding
- GO:0007411 axon guidance
- GO:0030235 nitric-oxide synthase regulator activity
- GO:0030911 TPR domain binding
- GO:0034619 cellular chaperone-mediated protein complex assembly
- GO:0042470 melanosome
- GO:0042803 protein homodimerization activity
- GO:0045040 protein import into mitochondrial outer membrane
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0046209 nitric oxide metabolic process
- GO:0050999 regulation of nitric-oxide synthase activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Heat Shock Protein 90 Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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