Heat Shock Protein 72 antibody

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Mouse anti Heat Shock Protein 72

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00586 WB 100 µl
Mouse anti Human heat shock protein 72 antibody recognizes the heat shock 70 kDa protein 1A/1B, also known as HSP70-1/HSP70-2, HSP70.1/HSP70.2, dnaK-type molecular chaperone HSP70-1, epididymis secretory protein Li 103, or heat shock 70 kDa protein 1/2.

The HSP70 intronless gene encodes a 70 kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins (provided by RefSeq, Jul 2008).

Mouse anti Human heat shock protein 72 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti heat shock protein 72 detects a band of approximately 70 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
Buffer Solution
HEPES buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
E. coli-derived recombinant human heat shock protein 72
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

More Information

UniProt
P0DMV8 Related reagents
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Heat Shock Protein 72 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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