Heat Shock Protein 72 antibody

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Anti Heat Shock Protein 72 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human heat shock protein 72 antibody (**VMA00586**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to heat shock protein 72 (molecular weight 70 kDa).

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Mouse anti Heat Shock Protein 72

Product Type: PrecisionAb Monoclonal
Isotype: IgG2b
Format: Purified
Specificity: Heat Shock Protein 72

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VMA00586 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human heat shock protein 72 antibody recognizes the heat shock 70 kDa protein 1A/1B, also known as HSP70-1/HSP70-2, HSP70.1/HSP70.2, dnaK-type molecular chaperone HSP70-1, epididymis secretory protein Li 103, or heat shock 70 kDa protein 1/2.

The HSP70 intronless gene encodes a 70 kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins (provided by RefSeq, Jul 2008).

Mouse anti Human heat shock protein 72 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti heat shock protein 72 detects a band of approximately 70 kDa in HEK293 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites.
Buffer Solution: HEPES buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen: E. coli-derived recombinant human heat shock protein 72
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.