Heat Shock Protein 70 antibody
Mouse anti Heat Shock Protein 70
Heat shock protein 70 antibody recognizes heat shock 70 kDa protein 1A/1B, also known as heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2 or HSP70.1/HSP70.2. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| VMA00042 | WB | 100 µl |
|
Mouse anti Human heat shock protein 70 antibody recognizes human heat shock protein 70 (HSP70), also known as heat shock 70 kDa protein 1A/1B, a 641 amino acid member of the HSP70 family espressed in the cytoplasm, which range in size from 66-78 kDa.
Heat shock proteins are a group of heat- or stress-inducible proteins which act as molecular chaperones and as essential regulators of cellular processes, including protein folding. HSP70 acts alongside other chaperones to mediate the folding of newly-synthesized peptide sequences, stabilize proteins against aggregation and assist in protein transmembrane transport.
Mouse anti Human heat shock protein 70 antibody recognizes human HSP70 as a single band of ~70 kDa in multiple human cell line lysates.
Heat shock proteins are a group of heat- or stress-inducible proteins which act as molecular chaperones and as essential regulators of cellular processes, including protein folding. HSP70 acts alongside other chaperones to mediate the folding of newly-synthesized peptide sequences, stabilize proteins against aggregation and assist in protein transmembrane transport.
Mouse anti Human heat shock protein 70 antibody recognizes human HSP70 as a single band of ~70 kDa in multiple human cell line lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti heat shock protein 70 antibody detects a band of approximately 70 kDa in HeLa cell lysates.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant human HSP70, amino acids 1-641, purified from E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P08107 Related reagents
- Entrez Gene
- HSPA1B Related reagents
- GO Terms
- GO:0044183 protein binding involved in protein folding
- GO:0051082 unfolded protein binding
- GO:0030529 ribonucleoprotein complex
- GO:0006986 response to unfolded protein
- GO:0042026 protein refolding
- GO:0005739 mitochondrion
- GO:0005829 cytosol
- GO:0005783 endoplasmic reticulum
- GO:0005524 ATP binding
- GO:0006402 mRNA catabolic process
- GO:0006916 anti-apoptosis
- GO:0008285 negative regulation of cell proliferation
- GO:0016234 inclusion body
- GO:0016607 nuclear speck
- GO:0030308 negative regulation of cell growth
- GO:0031625 ubiquitin protein ligase binding
- GO:0047485 protein N-terminus binding
- GO:0048471 perinuclear region of cytoplasm
- GO:0090084 negative regulation of inclusion body assembly
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Heat Shock Protein 70 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies
Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.
