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Heat Shock Protein 70 antibody

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Western Blot Validated
Anti Heat Shock Protein 70 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with heat shock protein 70 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to heat shock protein 70 (molecular weight 70 kDa)

Mouse anti Heat Shock Protein 70

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00042
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human heat shock protein 70 antibody recognizes human heat shock protein 70 (HSP70), also known as heat shock 70 kDa protein 1A/1B, a 641 amino acid member of the HSP70 family espressed in the cytoplasm, which range in size from 66-78 kDa.

Heat shock proteins are a group of heat- or stress-inducible proteins which act as molecular chaperones and as essential regulators of cellular processes, including protein folding. HSP70 acts alongside other chaperones to mediate the folding of newly-synthesized peptide sequences, stabilize proteins against aggregation and assist in protein transmembrane transport.

Mouse anti Human heat shock protein 70 antibody recognizes human HSP70 as a single band of ~70 kDa in multiple human cell line lysates.

Product Details

Target Species
Human
Western Blotting
Anti heat shock protein 70 antibody detects a band of approximately 70 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human HSP70, amino acids 1-641, purified from E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P08107
Entrez Gene
HSPA1B
GO Terms
GO:0044183 protein binding involved in protein folding
GO:0051082 unfolded protein binding
GO:0030529 ribonucleoprotein complex
GO:0006986 response to unfolded protein
GO:0042026 protein refolding
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005783 endoplasmic reticulum
GO:0005524 ATP binding
GO:0006402 mRNA catabolic process
GO:0006916 anti-apoptosis
GO:0008285 negative regulation of cell proliferation
GO:0016234 inclusion body
GO:0016607 nuclear speck
GO:0030308 negative regulation of cell growth
GO:0031625 ubiquitin protein ligase binding
GO:0047485 protein N-terminus binding
GO:0048471 perinuclear region of cytoplasm
GO:0090084 negative regulation of inclusion body assembly
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Heat Shock Protein 70 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
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