Heat Shock Protein 70 antibody
Mouse anti Heat Shock Protein 70
Heat shock protein 70 antibody recognizes heat shock 70 kDa protein 1A/1B, also known as heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2 or HSP70.1/HSP70.2. The antibody has been extensively validated for western blotting using whole cell lysates.
Heat Shock Protein 70 Antibody with Control Lysate
Heat shock protein 70 antibody recognizes heat shock 70 kDa protein 1A/1B, also known as heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2 or HSP70.1/HSP70.2. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00042 | WB | 100 µl | ||||
| VMA00042KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human heat shock protein 70 antibody recognizes human heat shock protein 70 (HSP70), also known as heat shock 70 kDa protein 1A/1B, a 641 amino acid member of the HSP70 family espressed in the cytoplasm, which range in size from 66-78 kDa.
Heat shock proteins are a group of heat- or stress-inducible proteins which act as molecular chaperones and as essential regulators of cellular processes, including protein folding. HSP70 acts alongside other chaperones to mediate the folding of newly-synthesized peptide sequences, stabilize proteins against aggregation and assist in protein transmembrane transport.
Mouse anti Human heat shock protein 70 antibody recognizes human HSP70 as a single band of ~70 kDa in multiple human cell line lysates.
Heat shock proteins are a group of heat- or stress-inducible proteins which act as molecular chaperones and as essential regulators of cellular processes, including protein folding. HSP70 acts alongside other chaperones to mediate the folding of newly-synthesized peptide sequences, stabilize proteins against aggregation and assist in protein transmembrane transport.
Mouse anti Human heat shock protein 70 antibody recognizes human HSP70 as a single band of ~70 kDa in multiple human cell line lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti heat shock protein 70 antibody detects a band of approximately 70 kDa in HeLa cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant human HSP70, amino acids 1-641, purified from E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P08107 Related reagents
- Entrez Gene
- HSPA1B Related reagents
- GO Terms
- GO:0044183 protein binding involved in protein folding
- GO:0051082 unfolded protein binding
- GO:0030529 ribonucleoprotein complex
- GO:0006986 response to unfolded protein
- GO:0042026 protein refolding
- GO:0005739 mitochondrion
- GO:0005829 cytosol
- GO:0005783 endoplasmic reticulum
- GO:0005524 ATP binding
- GO:0006402 mRNA catabolic process
- GO:0006916 anti-apoptosis
- GO:0008285 negative regulation of cell proliferation
- GO:0016234 inclusion body
- GO:0016607 nuclear speck
- GO:0030308 negative regulation of cell growth
- GO:0031625 ubiquitin protein ligase binding
- GO:0047485 protein N-terminus binding
- GO:0048471 perinuclear region of cytoplasm
- GO:0090084 negative regulation of inclusion body assembly
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Heat Shock Protein 70 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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