Heat Shock Protein 70 antibody

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Mouse anti Heat Shock Protein 70

Heat shock protein 70 antibody recognizes heat shock 70 kDa protein 1A/1B, also known as heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2 or HSP70.1/HSP70.2. The antibody has been extensively validated for western blotting using whole cell lysates.

Heat Shock Protein 70 Antibody with Control Lysate

Heat shock protein 70 antibody recognizes heat shock 70 kDa protein 1A/1B, also known as heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2 or HSP70.1/HSP70.2. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00042 WB 100 µl
VMA00042KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human heat shock protein 70 antibody recognizes human heat shock protein 70 (HSP70), also known as heat shock 70 kDa protein 1A/1B, a 641 amino acid member of the HSP70 family espressed in the cytoplasm, which range in size from 66-78 kDa.

Heat shock proteins are a group of heat- or stress-inducible proteins which act as molecular chaperones and as essential regulators of cellular processes, including protein folding. HSP70 acts alongside other chaperones to mediate the folding of newly-synthesized peptide sequences, stabilize proteins against aggregation and assist in protein transmembrane transport.

Mouse anti Human heat shock protein 70 antibody recognizes human HSP70 as a single band of ~70 kDa in multiple human cell line lysates.

Product Details

Target Species
Human
Western Blotting
Anti heat shock protein 70 antibody detects a band of approximately 70 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human HSP70, amino acids 1-641, purified from E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P08107 Related reagents
Entrez Gene
HSPA1B Related reagents
GO Terms
GO:0044183 protein binding involved in protein folding
GO:0051082 unfolded protein binding
GO:0030529 ribonucleoprotein complex
GO:0006986 response to unfolded protein
GO:0042026 protein refolding
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005783 endoplasmic reticulum
GO:0005524 ATP binding
GO:0006402 mRNA catabolic process
GO:0006916 anti-apoptosis
GO:0008285 negative regulation of cell proliferation
GO:0016234 inclusion body
GO:0016607 nuclear speck
GO:0030308 negative regulation of cell growth
GO:0031625 ubiquitin protein ligase binding
GO:0047485 protein N-terminus binding
GO:0048471 perinuclear region of cytoplasm
GO:0090084 negative regulation of inclusion body assembly
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Heat Shock Protein 70 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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