Heat Shock Protein 70 antibody | 40000000

Mouse anti Heat Shock Protein 70

Product Type
PrecisionAb Monoclonal
Clone
40000000
Isotype
IgG1
Format
Purified
Specificity
Heat Shock Protein 70

Product Code Applications Pack Size List Price Your Price Qty
VMA00042
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P WB 100 µl loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human heat shock protein 70 antibody recognizes human heat shock protein 70 (HSP70), also known as heat shock 70 kDa protein 1A/1B, a 641 amino acid member of the HSP70 family espressed in the cytoplasm, which range in size from 66-78 kDa.

Heat shock proteins are a group of heat- or stress-inducible proteins which act as molecular chaperones and as essential regulators of cellular processes, including protein folding. HSP70 acts alongside other chaperones to mediate the folding of newly-synthesized peptide sequences, stabilize proteins against aggregation and assist in protein transmembrane transport.

Mouse anti Human heat shock protein 70 antibody recognizes human HSP70 as a single band of ~70 kDa in multiple human cell line lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti heat shock protein 70 antibody detects a band of approximately 70 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant human HSP70, amino acids 1-641, purified from E. coli
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

References for Heat Shock Protein 70 antibody

  1. Dalli, J. et al. (2013) Heterogeneity in neutrophil microparticles reveals distinct proteome and functional properties.
    Mol Cell Proteomics. 12 (8): 2205-19.

Further Reading

  1. Tavaria, M. et al. (1996) A hitchhiker's guide to the human Hsp70 family.
    Cell Stress Chaperones. 1 (1): 23-8.

Synonyms
HSP70
UniProt
P08107
Entrez Gene
HSPA1B
GO Terms
GO:0044183 protein binding involved in protein folding
GO:0051082 unfolded protein binding
GO:0030529 ribonucleoprotein complex
GO:0006986 response to unfolded protein
GO:0042026 protein refolding
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005783 endoplasmic reticulum
GO:0005524 ATP binding
GO:0006402 mRNA catabolic process
GO:0006916 anti-apoptosis
GO:0008285 negative regulation of cell proliferation
GO:0016234 inclusion body
GO:0016607 nuclear speck
GO:0030308 negative regulation of cell growth
GO:0031625 ubiquitin protein ligase binding
GO:0047485 protein N-terminus binding
GO:0048471 perinuclear region of cytoplasm
GO:0090084 negative regulation of inclusion body assembly