Heat Shock Protein 60 antibody

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Mouse anti Heat Shock Protein 60

Heat shock protein 60 antibody recognizes mitochondrial 60 kDa heat shock protein, also known as 60 kDa chaperonin, HSP-60, Hsp60 HuCHA60 Mitochondrial matrix protein, or P1 P60 lymphocyte protein. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00041 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human heat shock protein 60 antibody recognizes human heat shock protein 60 (HSP60), a member of the highly conserved chaperonin (HSP60) family, expressed abundantly in the mitochondrial matrix and is closely related to E. coli GroEL and plant Rubisco binding protein chaperones.

Heat shock proteins are a group of heat- or stress-inducible proteins which act as molecular chaperones and as essential regulators of cellular processes, including protein folding. HSP60 acts in conjunction with the co-chaperonin HSP10, and is important in the refolding and transportation of proteins from the cytoplasm into the mitochondrial matrix, and also involved in the replication of mitochondrial DNA (Höhfeld et al. 1994).

Mouse anti Human heat shock protein 60 recognizes human HSP60 as a single band of ~60 kDa by western blotting under reducing conditions in multiple cell line lysates.

Product Details

Target Species

Human

Western Blotting

Anti heat shock protein 60 antibody detects a band of approximately 60 kDa in Raji cell lysates.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography on Protein G ascites

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Recombinant human Hsp60, amino acids 1-573, purified from E. coli

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: P10809 Related reagents
Entrez Gene: HSPD1 Related reagents
GO Terms: GO:0051082 unfolded protein binding; GO:0001530 lipopolysaccharide binding; GO:0002039 p53 binding; GO:0002368 B cell cytokine production; GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002842 positive regulation of T cell mediated immune response to tumor cell; GO:0003688 DNA replication origin binding; GO:0003697 single-stranded DNA binding; GO:0051087 chaperone binding; GO:0006986 response to unfolded protein; GO:0042026 protein refolding; GO:0005829 cytosol; GO:0005524 ATP binding; GO:0005615 extracellular space; GO:0005743 mitochondrial inner membrane; GO:0005759 mitochondrial matrix; GO:0005769 early endosome; GO:0030141 stored secretory granule; GO:0005905 coated pit; GO:0006458 'de novo' protein folding; GO:0051131 chaperone-mediated protein complex assembly; GO:0006919 activation of caspase activity; GO:0016887 ATPase activity; GO:0009986 cell surface; GO:0050821 protein stabilization; GO:0030135 coated vesicle; GO:0032727 positive regulation of interferon-alpha production; GO:0032729 positive regulation of interferon-gamma production; GO:0032733 positive regulation of interleukin-10 production; GO:0032735 positive regulation of interleukin-12 production; GO:0032755 positive regulation of interleukin-6 production; GO:0042100 B cell proliferation; GO:0042110 T cell activation; GO:0043032 positive regulation of macrophage activation; GO:0043065 positive regulation of apoptosis; GO:0043066 negative regulation of apoptosis; GO:0043498 cell surface binding; GO:0044419 interspecies interaction between organisms; GO:0046696 lipopolysaccharide receptor complex; GO:0048291 isotype switching to IgG isotypes; GO:0050870 positive regulation of T cell activation; GO:0051604 protein maturation
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Heat Shock Protein 60 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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