Heat Shock Protein 60 antibody

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Mouse anti Heat Shock Protein 60

Heat shock protein 60 antibody recognizes mitochondrial 60 kDa heat shock protein, also known as 60 kDa chaperonin, HSP-60, Hsp60 HuCHA60 Mitochondrial matrix protein, or P1 P60 lymphocyte protein. The antibody has been extensively validated for western blotting using whole cell lysates.

Heat Shock Protein 60 Antibody with Control Lysate

Heat shock protein 60 antibody recognizes mitochondrial 60 kDa heat shock protein, also known as 60 kDa chaperonin, chaperonin 60, CPN60 heat shock protein 60, HSP-60, Hsp60 HuCHA60 Mitochondrial matrix protein, or P1 P60 lymphocyte protein.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00041 WB 100 µl
VMA00041KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human heat shock protein 60 antibody recognizes human heat shock protein 60 (HSP60), a member of the highly conserved chaperonin (HSP60) family, expressed abundantly in the mitochondrial matrix and is closely related to E. coli GroEL and plant Rubisco binding protein chaperones.

Heat shock proteins are a group of heat- or stress-inducible proteins which act as molecular chaperones and as essential regulators of cellular processes, including protein folding. HSP60 acts in conjunction with the co-chaperonin HSP10, and is important in the refolding and transportation of proteins from the cytoplasm into the mitochondrial matrix, and also involved in the replication of mitochondrial DNA (Höhfeld et al. 1994).

Mouse anti Human heat shock protein 60 recognizes human HSP60 as a single band of ~60 kDa by western blotting under reducing conditions in multiple cell line lysates.

Product Details

Target Species
Human
Western Blotting
Anti heat shock protein 60 antibody detects a band of approximately 60 kDa in Raji cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human Hsp60, amino acids 1-573, purified from E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Raji lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P10809 Related reagents
Entrez Gene
HSPD1 Related reagents
GO Terms
GO:0051082 unfolded protein binding
GO:0001530 lipopolysaccharide binding
GO:0002039 p53 binding
GO:0002368 B cell cytokine production
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002842 positive regulation of T cell mediated immune response to tumor cell
GO:0003688 DNA replication origin binding
GO:0003697 single-stranded DNA binding
GO:0051087 chaperone binding
GO:0006986 response to unfolded protein
GO:0042026 protein refolding
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0005615 extracellular space
GO:0005743 mitochondrial inner membrane
GO:0005759 mitochondrial matrix
GO:0005769 early endosome
GO:0030141 stored secretory granule
GO:0005905 coated pit
GO:0006458 'de novo' protein folding
GO:0051131 chaperone-mediated protein complex assembly
GO:0006919 activation of caspase activity
GO:0016887 ATPase activity
GO:0009986 cell surface
GO:0050821 protein stabilization
GO:0030135 coated vesicle
GO:0032727 positive regulation of interferon-alpha production
GO:0032729 positive regulation of interferon-gamma production
GO:0032733 positive regulation of interleukin-10 production
GO:0032735 positive regulation of interleukin-12 production
GO:0032755 positive regulation of interleukin-6 production
GO:0042100 B cell proliferation
GO:0042110 T cell activation
GO:0043032 positive regulation of macrophage activation
GO:0043065 positive regulation of apoptosis
GO:0043066 negative regulation of apoptosis
GO:0043498 cell surface binding
GO:0044419 interspecies interaction between organisms
GO:0046696 lipopolysaccharide receptor complex
GO:0048291 isotype switching to IgG isotypes
GO:0050870 positive regulation of T cell activation
GO:0051604 protein maturation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Heat Shock Protein 60 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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