Heat Shock Protein 60 antibody
Mouse anti Heat Shock Protein 60
Heat shock protein 60 antibody recognizes mitochondrial 60 kDa heat shock protein, also known as 60 kDa chaperonin, HSP-60, Hsp60 HuCHA60 Mitochondrial matrix protein, or P1 P60 lymphocyte protein. The antibody has been extensively validated for western blotting using whole cell lysates.
Heat Shock Protein 60 Antibody with Control Lysate
Heat shock protein 60 antibody recognizes mitochondrial 60 kDa heat shock protein, also known as 60 kDa chaperonin, chaperonin 60, CPN60 heat shock protein 60, HSP-60, Hsp60 HuCHA60 Mitochondrial matrix protein, or P1 P60 lymphocyte protein.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00041 | WB | 100 µl | ||||
| VMA00041KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human heat shock protein 60 antibody recognizes human heat shock protein 60 (HSP60), a member of the highly conserved chaperonin (HSP60) family, expressed abundantly in the mitochondrial matrix and is closely related to E. coli GroEL and plant Rubisco binding protein chaperones.
Heat shock proteins are a group of heat- or stress-inducible proteins which act as molecular chaperones and as essential regulators of cellular processes, including protein folding. HSP60 acts in conjunction with the co-chaperonin HSP10, and is important in the refolding and transportation of proteins from the cytoplasm into the mitochondrial matrix, and also involved in the replication of mitochondrial DNA (Höhfeld et al. 1994).
Mouse anti Human heat shock protein 60 recognizes human HSP60 as a single band of ~60 kDa by western blotting under reducing conditions in multiple cell line lysates.
Heat shock proteins are a group of heat- or stress-inducible proteins which act as molecular chaperones and as essential regulators of cellular processes, including protein folding. HSP60 acts in conjunction with the co-chaperonin HSP10, and is important in the refolding and transportation of proteins from the cytoplasm into the mitochondrial matrix, and also involved in the replication of mitochondrial DNA (Höhfeld et al. 1994).
Mouse anti Human heat shock protein 60 recognizes human HSP60 as a single band of ~60 kDa by western blotting under reducing conditions in multiple cell line lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti heat shock protein 60 antibody detects a band of approximately 60 kDa in Raji cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant human Hsp60, amino acids 1-573, purified from E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Raji lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P10809 Related reagents
- Entrez Gene
- HSPD1 Related reagents
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0001530 lipopolysaccharide binding
- GO:0002039 p53 binding
- GO:0002368 B cell cytokine production
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002842 positive regulation of T cell mediated immune response to tumor cell
- GO:0003688 DNA replication origin binding
- GO:0003697 single-stranded DNA binding
- GO:0051087 chaperone binding
- GO:0006986 response to unfolded protein
- GO:0042026 protein refolding
- GO:0005829 cytosol
- GO:0005524 ATP binding
- GO:0005615 extracellular space
- GO:0005743 mitochondrial inner membrane
- GO:0005759 mitochondrial matrix
- GO:0005769 early endosome
- GO:0030141 stored secretory granule
- GO:0005905 coated pit
- GO:0006458 'de novo' protein folding
- GO:0051131 chaperone-mediated protein complex assembly
- GO:0006919 activation of caspase activity
- GO:0016887 ATPase activity
- GO:0009986 cell surface
- GO:0050821 protein stabilization
- GO:0030135 coated vesicle
- GO:0032727 positive regulation of interferon-alpha production
- GO:0032729 positive regulation of interferon-gamma production
- GO:0032733 positive regulation of interleukin-10 production
- GO:0032735 positive regulation of interleukin-12 production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0042100 B cell proliferation
- GO:0042110 T cell activation
- GO:0043032 positive regulation of macrophage activation
- GO:0043065 positive regulation of apoptosis
- GO:0043066 negative regulation of apoptosis
- GO:0043498 cell surface binding
- GO:0044419 interspecies interaction between organisms
- GO:0046696 lipopolysaccharide receptor complex
- GO:0048291 isotype switching to IgG isotypes
- GO:0050870 positive regulation of T cell activation
- GO:0051604 protein maturation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Heat Shock Protein 60 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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