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Heat Shock 70 Kda Protein 5 antibody

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Western Blot Validated
Anti Heat Shock 70 Kda Protein 5 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with heat shock 70 kDa protein 5 antibody followed by detection with Goat anti Human IgG F(ab')2:HRP (1/2,500, STAR126P) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to heat shock 70 kDa protein 5 (molecular weight 78 kDa)

Human anti Heat Shock 70 Kda Protein 5

Product Type
PrecisionAb Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00443
Datasheet
WB
MSDS
100 µl loader

Human anti Human heat shock protein 5 antibody recognizes heat shock protein 5 also known as 78 kDa glucose-regulated protein, GRP78, endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, BIP, epididymis secretory sperm binding protein Li 89n, immunoglobulin heavy chain-binding protein and MIF2.

Encoded by the HSPA5 gene and a member of the heat shock protein 70 (HSP70) family, heat shock protein 5 is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell (provided by RefSeq, Sep 2010).

Human anti Human heat shock protein 5 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti heat shock 70 kDa protein 5 detects a band of approximately 78 kDa in HepG2 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human heat Shock 70 kDa protein 5

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P11021 Related reagents
Entrez Gene
HSPA5 Related reagents
GO Terms
GO:0051082 unfolded protein binding
GO:0005509 calcium ion binding
GO:0002576 platelet degranulation
GO:0051087 chaperone binding
GO:0030674 protein binding, bridging
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005788 endoplasmic reticulum lumen
GO:0005793 ER-Golgi intermediate compartment
GO:0006916 anti-apoptosis
GO:0009986 cell surface
GO:0030168 platelet activation
GO:0030176 integral to endoplasmic reticulum membrane
GO:0030433 ER-associated protein catabolic process
GO:0030496 midbody
GO:0031625 ubiquitin protein ligase binding
GO:0042149 cellular response to glucose starvation
GO:0042470 melanosome
GO:0043027 caspase inhibitor activity
GO:0043154 negative regulation of caspase activity
GO:0048471 perinuclear region of cytoplasm
GO:0060904 regulation of protein folding in endoplasmic reticulum
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of Heat Shock 70 Kda Protein 5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader

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