Heat Shock 70 Kda Protein 5 antibody

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Anti Heat Shock 70 Kda Protein 5 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with heat shock 70 kDa protein 5 antibody followed by detection with Goat anti Human IgG F(ab')2:HRP (1/2,500, STAR126P) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to heat shock 70 kDa protein 5 (molecular weight 78 kDa)

Human anti Heat Shock 70 Kda Protein 5

Product Type: PrecisionAb Monoclonal
Isotype: HuCAL Fab bivalent
Format: Purified
Specificity: Heat Shock 70 Kda Protein 5

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Human anti Human heat shock protein 5 antibody recognizes heat shock protein 5 also known as 78 kDa glucose-regulated protein, GRP78, endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, BIP, epididymis secretory sperm binding protein Li 89n, immunoglobulin heavy chain-binding protein and MIF2.

Encoded by the HSPA5 gene and a member of the heat shock protein 70 (HSP70) family, heat shock protein 5 is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell (provided by RefSeq, Sep 2010).

Human anti Human heat shock protein 5 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti heat shock 70 kDa protein 5 detects a band of approximately 78 kDa in HepG2 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation: Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Recombinant human heat Shock 70 kDa protein 5
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

Target Species	Cross Reactivity
Mouse

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Human IgG F(ab')2:HRP	STAR126P	E WB	0.5 ml	Log in	Get Quote
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Description	Goat anti Human IgG F(ab')2:HRP

UniProt: P11021
Entrez Gene: HSPA5
GO Terms: GO:0051082 unfolded protein binding; GO:0005509 calcium ion binding; GO:0002576 platelet degranulation; GO:0051087 chaperone binding; GO:0030674 protein binding, bridging; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0005788 endoplasmic reticulum lumen; GO:0005793 ER-Golgi intermediate compartment; View More GO Terms; GO:0006916 anti-apoptosis; GO:0009986 cell surface; GO:0030168 platelet activation; GO:0030176 integral to endoplasmic reticulum membrane; GO:0030433 ER-associated protein catabolic process; GO:0030496 midbody; GO:0031625 ubiquitin protein ligase binding; GO:0042149 cellular response to glucose starvation; GO:0042470 melanosome; GO:0043027 caspase inhibitor activity; GO:0043154 negative regulation of caspase activity; GO:0048471 perinuclear region of cytoplasm; GO:0060904 regulation of protein folding in endoplasmic reticulum