Heat Shock 70 Kda Protein 5 antibody
Human anti Heat Shock 70 Kda Protein 5
Anti human heat shock 70 kDa protein 5 antibody recognizes HSPA5, also known as glucose regulated protein, 78 kDa, GRP78, BIP or epididymis secretory sperm binding protein Li 89n. This antibody has been extensively validated for western blotting using whole cell lysates.
Heat Shock 70 Kda Protein 5 Antibody with Control Lysate
Anti human heat shock 70 kDa protein 5 antibody recognizes HSPA5, also known as glucose regulated protein, 78 kDa, GRP78, BIP or epididymis secretory sperm binding protein Li 89n. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- HuCAL Fab bivalent
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00443 | WB | 100 µl | ||||
| VMA00443KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Human anti Human heat shock protein 5 antibody recognizes heat shock protein 5 also known as 78 kDa glucose-regulated protein, GRP78, endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, BIP, epididymis secretory sperm binding protein Li 89n, immunoglobulin heavy chain-binding protein and MIF2.
Encoded by the HSPA5 gene and a member of the heat shock protein 70 (HSP70) family, heat shock protein 5 is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell (provided by RefSeq, Sep 2010).
Human anti Human heat shock protein 5 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the HSPA5 gene and a member of the heat shock protein 70 (HSP70) family, heat shock protein 5 is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell (provided by RefSeq, Sep 2010).
Human anti Human heat shock protein 5 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti heat shock 70 kDa protein 5 detects a band of approximately 78 kDa in HepG2 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Product Form
- Purified IgG - liquid
- Preparation
- Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
- Preparation
- 20μl Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant human heat Shock 70 kDa protein 5
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HepG2 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P11021 Related reagents
- Entrez Gene
- HSPA5 Related reagents
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0005509 calcium ion binding
- GO:0002576 platelet degranulation
- GO:0051087 chaperone binding
- GO:0030674 protein binding, bridging
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0005788 endoplasmic reticulum lumen
- GO:0005793 ER-Golgi intermediate compartment
- GO:0006916 anti-apoptosis
- GO:0009986 cell surface
- GO:0030168 platelet activation
- GO:0030176 integral to endoplasmic reticulum membrane
- GO:0030433 ER-associated protein catabolic process
- GO:0030496 midbody
- GO:0031625 ubiquitin protein ligase binding
- GO:0042149 cellular response to glucose starvation
- GO:0042470 melanosome
- GO:0043027 caspase inhibitor activity
- GO:0043154 negative regulation of caspase activity
- GO:0048471 perinuclear region of cytoplasm
- GO:0060904 regulation of protein folding in endoplasmic reticulum
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb™ is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Heat Shock 70 Kda Protein 5 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | STAR126P | 0.5 ml | E WB | ||
| Goat anti Human IgG F(ab')2:HRP | STAR126P | 0.5 ml | E WB |
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