Find out more about A.I.den - our SearchBot

Heat Shock 70 Kda Protein 5 antibody

ISO 9001 Quality Assurance Manufacturing | Print
Anti Heat Shock 70 Kda Protein 5 Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1
Anti Heat Shock 70 Kda Protein 5 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with heat shock 70 kDa protein 5 antibody followed by detection with Goat anti Human IgG F(ab')2:HRP (1/2,500, STAR126P) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to heat shock 70 kDa protein 5 (molecular weight 78 kDa)

Human anti Heat Shock 70 Kda Protein 5

Product Type
PrecisionAb Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified
Specificity
Heat Shock 70 Kda Protein 5

Quick Links:

Antibody Quality and Performance Guarantee Antibody Quality and Performance GuaranteeAntibody Quality and
Performance Guarantee
Unrivaled technical support Unrivalled technical supportUnrivaled
Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
VMA00443
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Human anti Human heat shock protein 5 antibody recognizes heat shock protein 5 also known as 78 kDa glucose-regulated protein, GRP78, endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, BIP, epididymis secretory sperm binding protein Li 89n, immunoglobulin heavy chain-binding protein and MIF2.

Encoded by the HSPA5 gene and a member of the heat shock protein 70 (HSP70) family, heat shock protein 5 is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell (provided by RefSeq, Sep 2010).

Human anti Human heat shock protein 5 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti heat shock 70 kDa protein 5 detects a band of approximately 78 kDa in HepG2 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant human heat Shock 70 kDa protein 5
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader
List Price Your Price
loader
Description Goat anti Human IgG F(ab')2:HRP

UniProt
P11021
Entrez Gene
HSPA5
GO Terms
GO:0051082 unfolded protein binding
GO:0005509 calcium ion binding
GO:0002576 platelet degranulation
GO:0051087 chaperone binding
GO:0030674 protein binding, bridging
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005788 endoplasmic reticulum lumen
GO:0005793 ER-Golgi intermediate compartment
GO:0006916 anti-apoptosis
GO:0009986 cell surface
GO:0030168 platelet activation
GO:0030176 integral to endoplasmic reticulum membrane
GO:0030433 ER-associated protein catabolic process
GO:0030496 midbody
GO:0031625 ubiquitin protein ligase binding
GO:0042149 cellular response to glucose starvation
GO:0042470 melanosome
GO:0043027 caspase inhibitor activity
GO:0043154 negative regulation of caspase activity
GO:0048471 perinuclear region of cytoplasm
GO:0060904 regulation of protein folding in endoplasmic reticulum
View more products with Heat Shock 70 Kda Protein 5 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products