Heat Shock 70 Kda Protein 4 antibody

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Human anti Heat Shock 70 Kda Protein 4

Mouse anti human heat shock 70 kDa protein 4 antibody recognizes heat shock 70 kDa protein 4, also known as HSPA4, epididymis secretory sperm binding protein Li 5a, heat shock protein, 110 kDa or hsp70 RY. This antibody has been extensively validated for western blotting using whole cell lysates.

Heat Shock 70 Kda Protein 4 Antibody with Control Lysate

Mouse anti human heat shock 70 kDa protein 4 antibody recognizes heat shock 70 kDa protein 4, also known as HSPA4, epididymis secretory sperm binding protein Li 5a, heat shock 70-related protein APG-2, heat shock protein, 110 kDa or hsp70 RY.

Product Type
PrecisionAb™ Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00442 WB 100 µl
VMA00442KT WB 2 Westerns
With Lysate 400µg
Human anti Human heat shock 70 kDa protein 4 antibody recognizes heat shock 70 kDa protein 4, also known as HSPA4, epididymis secretory sperm binding protein Li 5a and heat shock 70-related protein APG-2.

Human anti Human heat shock 70 kDa protein 4 antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti heat shock 70 kDa protein 4 detects a band of approximately 110 kDa in MCF7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Product Form
Purified IgG - liquid
Preparation
Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
Preparation
20μl Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
GST-tagged heat shock 70 kDa protein 4 fusion protein
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg MCF7 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P34932 Related reagents
Entrez Gene
HSPA4 Related reagents
GO Terms
GO:0006986 response to unfolded protein
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0034619 cellular chaperone-mediated protein complex assembly
GO:0045040 protein import into mitochondrial outer membrane
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb™ is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Heat Shock 70 Kda Protein 4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB

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