Heat Shock 70 Kda Protein 4 antibody
Human anti Heat Shock 70 Kda Protein 4
Mouse anti human heat shock 70 kDa protein 4 antibody recognizes heat shock 70 kDa protein 4, also known as HSPA4, epididymis secretory sperm binding protein Li 5a, heat shock protein, 110 kDa or hsp70 RY. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- HuCAL Fab bivalent
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00442 | WB | 100 µl |
Human anti Human heat shock 70 kDa protein 4 antibody recognizes heat shock 70 kDa protein 4, also known as HSPA4, epididymis secretory sperm binding protein Li 5a and heat shock 70-related protein APG-2.
Human anti Human heat shock 70 kDa protein 4 antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Human anti Human heat shock 70 kDa protein 4 antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti heat shock 70 kDa protein 4 detects a band of approximately 110 kDa in MCF7 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- GST-tagged heat shock 70 kDa protein 4 fusion protein
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P34932 Related reagents
- Entrez Gene
- HSPA4 Related reagents
- GO Terms
- GO:0006986 response to unfolded protein
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0034619 cellular chaperone-mediated protein complex assembly
- GO:0045040 protein import into mitochondrial outer membrane
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb™ is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
- Regulatory
- For research purposes only
Applications of Heat Shock 70 Kda Protein 4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | STAR126P | 0.5 ml | E WB |
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