Heat Shock 70 Kda Protein 4 antibody
Human anti Human heat shock 70 kDa protein 4 antibody recognizes heat shock 70 kDa protein 4, also known as HSPA4, epididymis secretory sperm binding protein Li 5a and heat shock 70-related protein APG-2.
Human anti Human heat shock 70 kDa protein 4 antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Human anti Human heat shock 70 kDa protein 4 antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti heat shock 70 kDa protein 4 detects a band of approximately 110 kDa in MCF7 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- GST-tagged heat shock 70 kDa protein 4 fusion protein
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P34932
- Entrez Gene
- HSPA4
- GO Terms
- GO:0006986 response to unfolded protein
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0034619 cellular chaperone-mediated protein complex assembly
- GO:0045040 protein import into mitochondrial outer membrane
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
- Regulatory
- For research purposes only
Applications of Heat Shock 70 Kda Protein 4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | STAR126P | E WB | 0.5 ml |
|
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
