HbA1c antibody | AbD15783
* Data from triplicate experiments
1. Samples were obtained from heterozygous donors. The values reflect the mmol/mol of abnormal Hb carried by the donor.
The mmol/mol values were calculated using the IFCC standardization formula:
NGSP = [0.09148 x IFCC] + 2.152
* Data from triplicate experiments
Product Details
- Target Species
- Human
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- StrepTactin affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Affinity
- The affinity of this antibody, as measured against the immunogen, was measured as KD=19 nM by real time, label-free molecular interaction analysis using HbA1c-BSA and HbA1c-TRF conjugates as immobilized antigen.
The affinity of this antibody, as measured against purified HbA1c, was measured as KD=58 nM by real time, label-free molecular interaction analysis using full length HbA1c as immobilized antigen. - Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P68871
- Entrez Gene
- HBB
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of HbA1c antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product is suitable for use in direct and indirect ELISA applications.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti V5-Tag:Alk. Phos. | MCA1360A | E WB | 0.1 mg |
|
|
| Mouse anti V5-Tag:Biotin | MCA1360B | C E WB | 0.1 mg |
|
|
| Mouse anti V5-Tag:HRP | MCA1360P | E WB | 0.1 mg |
|
|
| Mouse anti Strep-Tag Classic:HRP | MCA2489P | E WB | 75 µl |
|
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