H1F0 antibody | CD03/1C11
Mouse anti H1F0 antibody recognizes histone H1.0, also known as H1-0. H1 linker histones are DNA-binding proteins involved with formation of higher-order chromatin structures, with epigenetic functions in DNA replication and repair, genome stability, and regulation of gene expression (Di Liegro et al. 2018). HIF0 is one of the variants of H1 but unlike replication-dependent H1 variants, H1F0 is expressed in dividing and non-dividing cells (Torres et al. 2016). H1F0 also differs from other histone H1 family members in the chromosomal location and organizing of the gene encoding it. The H1F0 variant is generally a lot shorter than other H1 variants (Kowalski et al. 2016). H1F0 has been found to bind to large numbers of proteins, suggesting an important role in protein-protein interactions beyond its functions in chromatic structure (Kalashnikova et al. 2013). Given that cancer development can be affected by epigenetic factors, it is unsurprising that histones are involved. Reversible silencing of H1F0 has been found to affect rhea differentiation state of cancer cells (Torres et al. 2016).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti H1F0 detects a band of approximately 30 kDa in A431 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-194 of human H1F0
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P07305
- Entrez Gene
- H1F0
- GO Terms
- GO:0000786 nucleosome
- GO:0003677 DNA binding
- GO:0006334 nucleosome assembly
- GO:0005654 nucleoplasm
- GO:0005794 Golgi apparatus
- GO:0006309 DNA fragmentation involved in apoptotic nuclear change
- GO:0015629 actin cytoskeleton
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of H1F0 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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