GSTA1 antibody

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Mouse anti GSTA1

Mouse anti human GSTA1 antibody recognizes glutathione S-transferase A1, also known as GSTA1, GST HA subunit 1, GST class-alpha member 1, GST, class alpha, 1 or GST-epsilon. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgM
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00356 WB 100 µl
Mouse anti Human GSTA1 antibody recognizes glutathione S-transferase A1, also known as GST HA subunit 1, GST class-alpha member 1, GST, class alpha, 1, GST-epsilon, S-(hydroxyalkyl)glutathione lyase A1, glutathione S-alkyltransferase A1, glutathione S-transferase 2 and glutathione S-transferase Ha subunit 1.

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs.

At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. The GSTA1 gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation (provided by RefSeq, Jul 2008).

Mouse anti Human GSTA1 antibody detects a band of 25 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti GSTA1 detects a band of approximately 25 kDa in mouse liver tissue
Product Form
Ig fraction - liquid
Preparation
Mouse monoclonal antibody purified from tissue culture supernatant by euglobulin precipitation
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant GSTA1 protein
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P08263 Related reagents
Entrez Gene
GSTA1 Related reagents
GO Terms
GO:0004364 glutathione transferase activity
GO:0005829 cytosol
GO:0006749 glutathione metabolic process
GO:0006805 xenobiotic metabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of GSTA1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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