GSK3 Alpha (pSer21) antibody

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Mouse anti GSK3 Alpha (pSer21)

GSK3 alpha (pSer21) antibody recognizes glycogen synthase kinase-3 (GSK3) alpha, when phosphorylated at Ser21.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00754 WB 100 µl
Mouse anti GSK3 alpha antibody recognizes glycogen synthase kinase-3 alpha, also known as serine/threonine-protein kinase GSK3A, when phosphorylated at serine 21. GSK3 controls several physiological processes, such as modulation of transcription factor AP-1 (Nikolakaki et al. 1993) and CREB (Fiol et al. 1994) as well as glycogen metabolism (Welsh and Proud 1993). The kinase has also been reported to regulate cyclin D1 proteolysis and localization (Diehl et al. 1998). Phosphorylation of GSK3 at serine 21 leads to inactivation of the kinase (Frame et al. 2001) and is associated with poor survival chances in Acute Myeloid Leukemia (AML) patients (Ruvolo et al. 2015).

Product Details

Target Species
Human
Western Blotting
Mouse anti GSK3 alpha (pSer21) detects a band of approximately 51 kDa in pervanadate-treated HepG2 (treated lysate) cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody purified by thiophillic adsorption and size exclusion chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
PEG & Sucrose
Immunogen
Phosphopeptide conjugated to KLH
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P49840 Related reagents
Entrez Gene
GSK3A Related reagents
GO Terms
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0008286 insulin receptor signaling pathway
GO:0010905 negative regulation of UDP-glucose catabolic process
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030877 beta-catenin destruction complex
GO:0034236 protein kinase A catalytic subunit binding
GO:0045732 positive regulation of protein catabolic process
GO:0046325 negative regulation of glucose import
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050321 tau-protein kinase activity
GO:0051348 negative regulation of transferase activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of GSK3 Alpha (pSer21) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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