GSK3 Alpha (pSer21) antibody | 9B8
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Mouse anti GSK3 alpha antibody, clone 9B8 recognizes glycogen synthase kinase-3 alpha, also known as serine/threonine-protein kinase GSK3A, when phosphorylated at serine 21. GSK3 controls several physiological processes, such as modulation of transcription factor AP-1 (Nikolakaki et al. 1993) and CREB (Fiol et al. 1994) as well as glycogen metabolism (Welsh and Proud 1993). The kinase has also been reported to regulate cyclin D1 proteolysis and localization (Diehl et al. 1998). Phosphorylation of GSK3 at serine 21 leads to inactivation of the kinase (Frame et al. 2001) and is associated with poor survival chances in Acute Myeloid Leukemia (AML) patients (Ruvolo et al. 2015).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti GSK3 alpha (pSer21) detects a band of approximately 51 kDa in pervanadate-treated HepG2 (treated lysate) cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody purified by thiophillic adsorption and size exclusion chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
PEG & Sucrose - Immunogen
- Phosphopeptide conjugated to KLH
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P49840
- Entrez Gene
- GSK3A
- GO Terms
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0005524 ATP binding
- GO:0006468 protein phosphorylation
- GO:0008286 insulin receptor signaling pathway
- GO:0010905 negative regulation of UDP-glucose catabolic process
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030877 beta-catenin destruction complex
- GO:0034236 protein kinase A catalytic subunit binding
- GO:0045732 positive regulation of protein catabolic process
- GO:0046325 negative regulation of glucose import
- GO:0046627 negative regulation of insulin receptor signaling pathway
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050321 tau-protein kinase activity
- GO:0051348 negative regulation of transferase activity
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of GSK3 Alpha (pSer21) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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