GSK3 Alpha (pSer21) antibody | 9B8

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Anti GSK3 Alpha (pSer21) Antibody, clone 9B8 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti GSK3 Alpha (pSer21) Antibody, clone 9B8 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti GSK3 Alpha (pSer21) Antibody, clone 9B8 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western Blot analysis of HepG2 untreated and pervanadate (PVD) treated whole cell lysates probed with (A) Rabbit anti GSK alpha antibody (VPA00205) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) or (B) Mouse anti GSK3 alpha (pSer21) antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P). Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a (A) 75 second or a (B) 63 second exposure. GSK3 alpha (pSer21) has a molecular weight of 51 kDa.

Anti GSK3 Alpha (pSer21) Antibody, clone 9B8 (PrecisionAb Monoclonal Antibody) gallery image 2 — Normalized signal intensity of GSK3 alpha (pSer21) from PVD treated and untreated lysates, treated with (+) or without (-) lambda protein phosphatase. Lambda protein phosphatase dephosphorylates serine, threonine and tyrosine residues. Normalized signal intensity was calculated by dividing the TPN-Normalized signal intensity for each phosphoprotein by the TPN-normalized intensity of the corresponding total protein.
Learn more about TPN Total Protein Normalization).

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Mouse anti GSK3 Alpha (pSer21)

Product Type: PrecisionAb Monoclonal
Clone: 9B8
Isotype: IgG1
Format: Purified
Specificity: GSK3 Alpha (pSer21)

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VMA00754 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti GSK3 alpha antibody, clone 9B8 recognizes glycogen synthase kinase-3 alpha, also known as serine/threonine-protein kinase GSK3A, when phosphorylated at serine 21. GSK3 controls several physiological processes, such as modulation of transcription factor AP-1 (Nikolakaki et al. 1993) and CREB (Fiol et al. 1994) as well as glycogen metabolism (Welsh and Proud 1993). The kinase has also been reported to regulate cyclin D1 proteolysis and localization (Diehl et al. 1998). Phosphorylation of GSK3 at serine 21 leads to inactivation of the kinase (Frame et al. 2001) and is associated with poor survival chances in Acute Myeloid Leukemia (AML) patients (Ruvolo et al. 2015).

Target Species: Human
Western Blotting: Mouse anti GSK3 alpha (pSer21) detects a band of approximately 51 kDa in pervanadate-treated HepG2 (treated lysate) cell lysates
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody purified by thiophillic adsorption and size exclusion chromatography from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
PEG & Sucrose
Immunogen: Phosphopeptide conjugated to KLH
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

Synonyms: GSK3A
UniProt: P49840
Entrez Gene: GSK3A
GO Terms: GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0005524 ATP binding; GO:0006468 protein phosphorylation; GO:0008286 insulin receptor signaling pathway; GO:0010905 negative regulation of UDP-glucose catabolic process; GO:0048015 phosphatidylinositol-mediated signaling; GO:0030877 beta-catenin destruction complex; GO:0034236 protein kinase A catalytic subunit binding; View More GO Terms; GO:0045732 positive regulation of protein catabolic process; GO:0046325 negative regulation of glucose import; GO:0046627 negative regulation of insulin receptor signaling pathway; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050321 tau-protein kinase activity; GO:0051348 negative regulation of transferase activity