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GSK3 Alpha (pSer21) antibody | 9B8

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Anti GSK3 Alpha (pSer21) Antibody, clone 9B8 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti GSK3 Alpha (pSer21) Antibody, clone 9B8 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti GSK3 Alpha (pSer21) Antibody, clone 9B8 (PrecisionAb Monoclonal Antibody) gallery image 1
Western Blot analysis of HepG2 untreated and pervanadate (PVD) treated whole cell lysates probed with (A) Rabbit anti GSK alpha antibody (VPA00205) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) or (B) Mouse anti GSK3 alpha (pSer21) antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P). Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a (A) 75 second or a (B) 63 second exposure. GSK3 alpha (pSer21) has a molecular weight of 51 kDa.
Anti GSK3 Alpha (pSer21) Antibody, clone 9B8 (PrecisionAb Monoclonal Antibody) gallery image 2
Normalized signal intensity of GSK3 alpha (pSer21) from PVD treated and untreated lysates, treated with (+) or without (-) lambda protein phosphatase. Lambda protein phosphatase dephosphorylates serine, threonine and tyrosine residues. Normalized signal intensity was calculated by dividing the TPN-Normalized signal intensity for each phosphoprotein by the TPN-normalized intensity of the corresponding total protein.
Learn more about TPN Total Protein Normalization).

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Mouse anti GSK3 Alpha (pSer21)

Product Type
PrecisionAb Monoclonal
Clone
9B8
Isotype
IgG1
Format
Purified
Specificity
GSK3 Alpha (pSer21)

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VMA00754
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Mouse anti GSK3 alpha antibody, clone 9B8 recognizes glycogen synthase kinase-3 alpha, also known as serine/threonine-protein kinase GSK3A, when phosphorylated at serine 21. GSK3 controls several physiological processes, such as modulation of transcription factor AP-1 (Nikolakaki et al. 1993) and CREB (Fiol et al. 1994) as well as glycogen metabolism (Welsh and Proud 1993). The kinase has also been reported to regulate cyclin D1 proteolysis and localization (Diehl et al. 1998). Phosphorylation of GSK3 at serine 21 leads to inactivation of the kinase (Frame et al. 2001) and is associated with poor survival chances in Acute Myeloid Leukemia (AML) patients (Ruvolo et al. 2015). PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti GSK3 alpha (pSer21) detects a band of approximately 51 kDa in pervanadate-treated HepG2 (treated lysate) cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody purified by thiophillic adsorption and size exclusion chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
PEG & Sucrose
Immunogen
Phosphopeptide conjugated to KLH
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

Synonyms
GSK3A
UniProt
P49840
Entrez Gene
GSK3A
GO Terms
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0008286 insulin receptor signaling pathway
GO:0010905 negative regulation of UDP-glucose catabolic process
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030877 beta-catenin destruction complex
GO:0034236 protein kinase A catalytic subunit binding
GO:0045732 positive regulation of protein catabolic process
GO:0046325 negative regulation of glucose import
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050321 tau-protein kinase activity
GO:0051348 negative regulation of transferase activity
View more products with GSK3 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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