GSK3 Alpha/Beta antibody

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Mouse anti GSK3 Alpha/Beta

GSK3 alpha /beta antibody recognizes glycogen synthase kinase-3 alpha also known as GSK-3 alpha or serine/threonine-protein kinase GSK3A and glycogen synthase kinase-3 beta, also known as GSK-3 beta or serine/threonine-protein kinase GSK3B.

GSK3 Alpha/Beta Antibody with Control Lysate

GSK3 alpha /beta antibody recognizes glycogen synthase kinase-3 alpha also known as GSK-3 alpha or serine/threonine-protein kinase GSK3A and glycogen synthase kinase-3 beta, also known as GSK-3 beta or serine/threonine-protein kinase GSK3B.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00342 WB 100 µl
VMA00342KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human GSK3 alpha/beta antibody recognizes glycogen synthase kinase-3 (GSK3) alpha also known as glycogen synthase kinase-3 alpha, GSK-3 alpha and serine/threonine-protein kinase GSK3A and the glycogen synthase kinase-3 (GSK3) beta, also known as GSK-3 beta, GSK3beta isoform, glycogen synthase kinase-3 beta and serine/threonine-protein kinase GSK3B.

The proteins encoded by GSK3A and GSK3B genes are a serine-threonine kinases, belonging to the glycogen synthase kinase subfamily. GSK3A is involved in Alzheimer and diabetes and GSK3B have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of GSK3B may be relevant to the pathogenesis of Alzheimer disease (provided by RefSeq, Sep 2009).

Mouse anti Human GSK3 alpha/beta antibody detects bands of 51 and 47 kDa respectively. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti GSK3 alpha/beta detect bands of approximately 47 kDa and 51 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 205-221 of human GSK3 beta
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95�C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P49840 Related reagents
P49841 Related reagents
Entrez Gene
GSK3A Related reagents
GSK3B Related reagents
GO Terms
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0008286 insulin receptor signaling pathway
GO:0010905 negative regulation of UDP-glucose catabolic process
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030877 beta-catenin destruction complex
GO:0034236 protein kinase A catalytic subunit binding
GO:0045732 positive regulation of protein catabolic process
GO:0046325 negative regulation of glucose import
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050321 tau-protein kinase activity
GO:0051348 negative regulation of transferase activity
GO:0001085 RNA polymerase II transcription factor binding
GO:0001837 epithelial to mesenchymal transition
GO:0001954 positive regulation of cell-matrix adhesion
GO:0002039 p53 binding
GO:0005634 nucleus
GO:0005977 glycogen metabolic process
GO:0006983 ER overload response
GO:0007411 axon guidance
GO:0008013 beta-catenin binding
GO:0018105 peptidyl-serine phosphorylation
GO:0021766 hippocampus development
GO:0031333 negative regulation of protein complex assembly
GO:0031334 positive regulation of protein complex assembly
GO:0031625 ubiquitin protein ligase binding
GO:0032091 negative regulation of protein binding
GO:0032855 positive regulation of Rac GTPase activity
GO:0034747 Axin-APC-beta-catenin-GSK3B complex
GO:0043066 negative regulation of apoptosis
GO:0046827 positive regulation of protein export from nucleus
GO:0051059 NF-kappaB binding
GO:0060070 canonical Wnt receptor signaling pathway
GO:0071109 superior temporal gyrus development
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb� is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of GSK3 Alpha/Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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