GSK3 Alpha/Beta antibody

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Mouse anti GSK3 Alpha/Beta

GSK3 alpha /beta antibody recognizes glycogen synthase kinase-3 alpha also known as GSK-3 alpha or serine/threonine-protein kinase GSK3A and glycogen synthase kinase-3 beta, also known as GSK-3 beta or serine/threonine-protein kinase GSK3B.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG2b
Format: Purified

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
VMA00342	WB			100 µl

Summary
Secondary Antibodies

Mouse anti Human GSK3 alpha/beta antibody recognizes glycogen synthase kinase-3 (GSK3) alpha also known as glycogen synthase kinase-3 alpha, GSK-3 alpha and serine/threonine-protein kinase GSK3A and the glycogen synthase kinase-3 (GSK3) beta, also known as GSK-3 beta, GSK3beta isoform, glycogen synthase kinase-3 beta and serine/threonine-protein kinase GSK3B.

The proteins encoded by GSK3A and GSK3B genes are a serine-threonine kinases, belonging to the glycogen synthase kinase subfamily. GSK3A is involved in Alzheimer and diabetes and GSK3B have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of GSK3B may be relevant to the pathogenesis of Alzheimer disease (provided by RefSeq, Sep 2009).

Mouse anti Human GSK3 alpha/beta antibody detects bands of 51 and 47 kDa respectively. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti GSK3 alpha/beta detect bands of approximately 47 kDa and 51 kDa in HEK293 cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Synthetic peptide corresponding to amino acids 205-221 of human GSK3 beta

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: P49840 Related reagents; P49841 Related reagents
Entrez Gene: GSK3A Related reagents; GSK3B Related reagents
GO Terms: GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0005524 ATP binding; GO:0006468 protein phosphorylation; GO:0008286 insulin receptor signaling pathway; GO:0010905 negative regulation of UDP-glucose catabolic process; GO:0048015 phosphatidylinositol-mediated signaling; GO:0030877 beta-catenin destruction complex; GO:0034236 protein kinase A catalytic subunit binding; GO:0045732 positive regulation of protein catabolic process; GO:0046325 negative regulation of glucose import; GO:0046627 negative regulation of insulin receptor signaling pathway; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050321 tau-protein kinase activity; GO:0051348 negative regulation of transferase activity; GO:0001085 RNA polymerase II transcription factor binding; GO:0001837 epithelial to mesenchymal transition; GO:0001954 positive regulation of cell-matrix adhesion; GO:0002039 p53 binding; GO:0005634 nucleus; GO:0005977 glycogen metabolic process; GO:0006983 ER overload response; GO:0007411 axon guidance; GO:0008013 beta-catenin binding; GO:0018105 peptidyl-serine phosphorylation; GO:0021766 hippocampus development; GO:0031333 negative regulation of protein complex assembly; GO:0031334 positive regulation of protein complex assembly; GO:0031625 ubiquitin protein ligase binding; GO:0032091 negative regulation of protein binding; GO:0032855 positive regulation of Rac GTPase activity; GO:0034747 Axin-APC-beta-catenin-GSK3B complex; GO:0043066 negative regulation of apoptosis; GO:0046827 positive regulation of protein export from nucleus; GO:0051059 NF-kappaB binding; GO:0060070 canonical Wnt receptor signaling pathway; GO:0071109 superior temporal gyrus development
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of GSK3 Alpha/Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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