GRB2 antibody | 32F3-G7

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Mouse anti GRB2

Product Type
PrecisionAb Monoclonal
Clone
32F3-G7
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00869
Datasheet
WB
SDS
100 µl loader

Mouse anti Human GRB2 antibody recognizes the Growth factor receptor-bound protein 2, also known as HT027, SH2/SH3 adapter GRB2, abundant SRC homology or epidermal growth factor receptor-binding protein GRB2.

The protein encoded by GRB2 gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. GRB2 is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for GRB2 (provided by RefSeq, Jul 2008).

Mouse anti Human GRB2 antibody detects a band of 25 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti GRB2 detects a band of approximately 25 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human GRB2
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P62993
Entrez Gene
GRB2
GO Terms
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0005070 SH3/SH2 adaptor activity
GO:0005154 epidermal growth factor receptor binding
GO:0005794 Golgi apparatus
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007267 cell-cell signaling
GO:0007411 axon guidance
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0010670 positive regulation of oxygen and reactive oxygen species metabolic process
GO:0031295 T cell costimulation
GO:0031623 receptor internalization
GO:0034329 cell junction assembly
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
GO:0042770 signal transduction in response to DNA damage
GO:0043560 insulin receptor substrate binding
GO:0044419 interspecies interaction between organisms
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050900 leukocyte migration
GO:0071479 cellular response to ionizing radiation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of GRB2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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