GRAP2 antibody | OTI1G2

Mouse anti GRAP2

Product Type
PrecisionAb Monoclonal
Clone
OTI1G2
Isotype
IgG1
Format
Purified
Specificity
GRAP2

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VMA00178
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IP WB 100 µl loader
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Mouse anti Human GRAP2 antibody, clone OTI1G2 recognizes GRB2-related adapter protein 2 (GRAP2), also known as GRB-2-like protein, GRB2-related protein with insert domain, SH3-SH2-SH3 adapter Mona, SH3-SH2-SH3 adaptor molecule, adapter protein GRID, grf-40, grf40 adapter protein, growth factor receptor-binding protein, growth factor receptor-bound protein 2-related adaptor protein 2, hematopoietic cell-associated adapter protein GrpL and hematopoietic cell-associated adaptor protein GRPL.

Encoded by the GRAP2 gene and a member of the GRB2/Sem5/Drk family, GRB2-related adapter protein 2 is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist (provided by RefSeq, Jul 2008).

Mouse anti Human GRAP2 antibody, clone OTI1G2 detects a band of 37 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti GRAP2 detects a band of approximately 37 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human GRAP2 (NP_004801) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
O75791
Entrez Gene
GRAP2
GO Terms
GO:0005829 cytosol
GO:0005070 SH3/SH2 adaptor activity
GO:0007265 Ras protein signal transduction
GO:0007267 cell-cell signaling
GO:0031295 T cell costimulation
GO:0050852 T cell receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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