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Granzyme A antibody | GA6

Mouse anti Human Granzyme A

Product Type
Monoclonal Antibody
Clone
GA6
Isotype
IgG1
Specificity
Granzyme A

Product Code Applications Pack Size List Price Your Price Qty
MCA2117
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 mg loader
List Price Your Price
loader

Mouse anti Human granzyme A antibody, clone GA6 recognizes Granzyme A, a ~60 kDa disulphide-linked homodimeric protein of two 262 amino acid chains, expressed in cytoplasmic granules of cytotoxic lymphocytes and NK cells.

Granzyme A is involved in the induction of apoptosis via its activity as a serine protease, but this would seem to be subsidiary to the role of Granzyme B. Granzyme A deficient mice are indistinguishable from normal animals in their response to infection.

Granzyme A has been proposed as a potential biomarker for patients with active tuberculosis with significantly lower levels present in the plasma of patients with the active form of the disease compared to patients with latent infection (Guggino et al. 2015).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
Recombinant human Granzyme A.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1/50 1/200
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for Granzyme A antibody

  1. Kummer, J.A. et al. (1993) Production and characterization of monoclonal antibodies raised against recombinant human granzymes A and B and showing cross reactions with the natural proteins.
    J Immunol Methods. 163 (1): 77-83.
  2. Mahrus, S. & Craik, C.S. (2005) Selective chemical functional probes of granzymes A and B reveal granzyme B is a major effector of natural killer cell-mediated lysis of target cells.
    Chem Biol. 12: 567-77.
  3. Suck, G. et al. (2005) KHYG-1, a model for the study of enhanced natural killer cell cytotoxicity.
    Exp Hematol. 33: 1160-71.
  4. Meade, J.L. et al. (2009) Proteolytic activation of the cytotoxic phenotype during human NK cell development.
    J Immunol. 183: 803-13.
  5. Hochegger, K. et al. (2007) Expression of granzyme A in human polymorphonuclear neutrophils.
    Immunology. 121: 166-73.
  6. Grodzovski, I. et al. (2011) IL-2-granzyme A chimeric protein overcomes multidrug resistance (MDR) through a caspase 3-independent apoptotic pathway.
    Int J Cancer. 128: 1966-80.
  7. Vrazo, A.C. et al. (2015) Live cell evaluation of granzyme delivery and death receptor signaling in tumor cells targeted by human natural killer cells.
    Blood. 126 (8): e1-e10.
  8. Korten, S. et al. (2008) Natural death of adult Onchocerca volvulus and filaricidal effects of doxycycline induce local FOXP3+/CD4+ regulatory T cells and granzyme expression.
    Microbes Infect. 10 (3): 313-24.
  9. View The Latest Product References

ELISA

Flow Cytometry

Western Blotting

RRID
AB_2263934
UniProt
P12544
Entrez Gene
GZMA
GO Terms
GO:0001772 immunological synapse
GO:0004252 serine-type endopeptidase activity
GO:0005576 extracellular region
GO:0005634 nucleus
GO:0006922 cleavage of lamin
GO:0006955 immune response
GO:0019835 cytolysis
GO:0032078 negative regulation of endodeoxyribonuclease activity
GO:0042803 protein homodimerization activity
GO:0043065 positive regulation of apoptosis
GO:0043392 negative regulation of DNA binding
GO:0051354 negative regulation of oxidoreductase activity

MCA2117

170706

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