Gnas antibody | OTI13D7

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Anti Gnas Antibody, clone OTI13D7 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Gnas Antibody, clone OTI13D7 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with GNAS antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 28 second exposure. Arrow points to GNAS (molecular weight 44 kDa)

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Mouse anti Gnas

Product Type: PrecisionAb Monoclonal
Clone: OTI13D7
Isotype: IgG2b
Format: Purified
Specificity: Gnas

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Mouse anti Human GNAS antibody recognizes GNAS, also known as adenylate cyclase-stimulating G alpha protein, guanine nucleotide-binding protein G(s) subunit alpha isoforms-short, alpha stimulating activity polypeptide 1 and guanine nucleotide regulatory protein.

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. Alternative splicing of downstream exons is observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for GNAS. Mutations in GNAS result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors (provided by RefSeq, Aug 2012).

Mouse anti Human GNAS antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Mouse anti GNAS detects a band of approximately 44 kDa in HepG2 cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from ascites
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen: HEK293-derived full length recombinant protein of human GNAS
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.