Mouse anti Human GNAS antibody recognizes GNAS, also known as adenylate cyclase-stimulating G alpha protein, guanine nucleotide-binding protein G(s) subunit alpha isoforms-short, alpha stimulating activity polypeptide 1 and guanine nucleotide regulatory protein.

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. Alternative splicing of downstream exons is observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for GNAS. Mutations in GNAS result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors (provided by RefSeq, Aug 2012).

Mouse anti Human GNAS antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000