GM-CSF antibody | OTI1A3

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Anti GM-CSF Antibody, clone OTI1A3 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti GM-CSF Antibody, clone OTI1A3 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human GM-CSF antibody, clone OTI1A3 (**VMA00213**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 299 second exposure. Arrow points to GM-CSF (molecular weight 25 kDa).

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Mouse anti GM-CSF

Product Type: PrecisionAb Monoclonal
Clone: OTI1A3
Isotype: IgG2b
Format: Purified
Specificity: GM-CSF

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00213 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human GM-CSF antibody, clone OTI1A3 recognizes granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as CSF, molgramostin and sargramostim.

Encoded by the CSF2 gene, GM-CSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. CSF2 has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13 (provided by RefSeq, Jul 2008).

Mouse anti Human GM-CSF antibody, clone OTI1A3 detects a band of 25 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti GM-CSF detects a band of approximately 25 kDa in Mouse thymus cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein corresponding to amino acids 18-144 of human GM-CSF (NP_000749) produced in E. coli
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P04141
Entrez Gene: CSF2
GO Terms: GO:0005125 cytokine activity; GO:0005129 granulocyte macrophage colony-stimulating factor receptor binding; GO:0005615 extracellular space; GO:0006955 immune response; GO:0008083 growth factor activity; GO:0010744 positive regulation of macrophage derived foam cell differentiation; GO:0032747 positive regulation of interleukin-23 production; GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein; GO:0045740 positive regulation of DNA replication; View More GO Terms; GO:0045918 negative regulation of cytolysis