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GM-CSF antibody | E01-1B6

Mouse anti Human GM-CSF

Product Type
Monoclonal Antibody
Clone
E01-1B6
Isotype
IgG2a
Specificity
GM-CSF

Product Code Applications Pack Size List Price Your Price Qty
MCA6230GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Mouse anti Human GM-CSF antibody, clone E01-1B6, recognizes Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) also known as colony stimulating factor 2. GM-CSF is a cytokine and acts as a hematopoietic grown factor and immune modulator of various immune cells. It is produced by immune cells including T cells and macrophages as well as endothelial and fibroblast cells. GM-CSF has various functions such as roles in recruiting circulating neutrophils, monocytes and lymphocytes (Shi et al. 2006). It is also involved in the maturation and activation of neutrophils and macrophages to induce microbial activity and the production of cytokines. GM-CSF elicits these functions through signaling via its receptor GM-CSFR to activate NF-kappaB or STAT5 signaling pathways (Dougan et al. 2019).

The purified Mouse anti Human GM-CSF antibody, clone E01-1B6 (MCA6230GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human GM-CSF antibody, clone E04-5B11 (MCA6231B) as the detection antibody.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli. derived recombinant human GM-CSF (Ala18-Glu144)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human GM-CSF:Biotin MCA6231B E 0.1 mg loader
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loader
Description Mouse anti Human GM-CSF:Biotin

UniProt
P04141
Entrez Gene
CSF2
GO Terms
GO:0005125 cytokine activity
GO:0005129 granulocyte macrophage colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0010744 positive regulation of macrophage derived foam cell differentiation
GO:0032747 positive regulation of interleukin-23 production
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0045740 positive regulation of DNA replication
GO:0045918 negative regulation of cytolysis

MCA6230GA

100005285

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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